1ux6: Difference between revisions

Thrombospondins (TSPs) are extracellular regulators of cell-matrix, interactions and cell phenotype. The most highly conserved region of all, TSPs are the calcium-binding type 3 (T3) repeats and the C-terminal, globular domain (CTD). The crystal structure of a cell-binding TSP-1, fragment, spanning three T3 repeats and the CTD, reveals a compact, assembly. The T3 repeats lack secondary structure and are organised around, a core of calcium ions; two DxDxDGxxDxxD motifs per repeat each, encapsulate two calcium ions in a novel arrangement. The CTD forms a, lectin-like beta-sandwich and contains four strictly conserved, calcium-binding sites. Disruption of the hairpin structure of T3 repeats 6, and 7 decreases protein secretion and stability. The availability for cell, attachment of an RGD motif in T3 repeat 7 is modulated by calcium loading., The central architectural role of calcium explains how it is critical for, the functions of the TSP C-terminal region. Mutations in the T3 repeats of, TSP-5/COMP, which cause two human skeletal disorders, are predicted to, disrupt the tertiary structure of the T3-CTD assembly.

1UX6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UX6 OCA].  

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