4v4a

Crystal Structure of the Wild Type Ribosome from E. Coli 70S Ribosome.Crystal Structure of the Wild Type Ribosome from E. Coli 70S Ribosome.

Structural highlights 4v4a is a 53 chain structure with sequence from Escherichia coli. This structure supersedes and combines the now removed PDB entries 1pnx and 1pny. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. NonStd Res: , Related: 1pnx, 1pny, 1pns, 1pnu Resources: FirstGlance, OCA, RCSB, PDBsum Warning: this is a large structure, and loading might take a long time or not happen at all. Publication Abstract from PubMed Protein biosynthesis on the ribosome requires accurate reading of the genetic code in mRNA. Two conformational rearrangements in the small ribosomal subunit, a closing of the head and body around the incoming tRNA and an RNA helical switch near the mRNA decoding site, have been proposed to select for complementary base-pairing between mRNA codons and tRNA anticodons. We determined x-ray crystal structures of the WT and a hyper-accurate variant of the Escherichia coli ribosome at resolutions of 10 and 9 A, respectively, revealing that formation of the intact 70S ribosome from its two subunits closes the conformation of the head of the small subunit independent of mRNA decoding. Moreover, no change in the conformation of the switch helix is observed in two steps of tRNA discrimination. These 70S ribosome structures indicate that mRNA decoding is coupled primarily to movement of the small subunit body, consistent with previous proposals, whereas closing of the head and the helical switch may function in other steps of protein synthesis. X-ray crystal structures of the WT and a hyper-accurate ribosome from Escherichia coli.,Vila-Sanjurjo A, Ridgeway WK, Seymaner V, Zhang W, Santoso S, Yu K, Cate JH Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8682-7. Epub 2003 Jul 9. PMID:12853578 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 4v4a, resolution 9.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image