7cat: Difference between revisions

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Revision as of 20:14, 20 March 2008

File:7cat.gif

, resolution 2.5Å

Ligands:

and

Activity:

Catalase, with EC number 1.11.1.6

Coordinates:

save as pdb, mmCIF, xml

THE NADPH BINDING SITE ON BEEF LIVER CATALASE

OverviewOverview

Beef liver and human erythrocyte catalases (EC 1.11.1.6) bind NADP tenaciously [Kirkman, H. N. & Gaetani, G. F. (1984) Proc. Natl. Acad. Sci. USA 81, 4343-4348]. The position of NADP on beef liver catalase corresponds to the carboxyl-terminal polypeptide hinge in Penicillium vitale fungal catalase, which connects the common catalase structure to the additional flavodoxin-like domain. In contrast to nearly all other known structures of protein-bound NADP, NAD, and FAD, the NADP molecule of beef liver catalase is folded into a right-handed helix and bound, in part, in the vicinity of the carboxyl end of two alpha-helices. A water molecule (W7) occupies a pseudosubstrate site close to the C4 position of the nicotinamide and is hydrogen bonded to His-304. Although the NADP and heme groups approach each other to within 13.7 A, there is no direct interaction. The function of the NADP remains a mystery.

About this StructureAbout this Structure

7CAT is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

ReferenceReference

The NADPH binding site on beef liver catalase., Fita I, Rossmann MG, Proc Natl Acad Sci U S A. 1985 Mar;82(6):1604-8. PMID:3856839

Page seeded by OCA on Thu Mar 20 19:14:20 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:7cat.gif|left|200px]]<br /><applet load="7cat" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:7cat.gif|left|200px]]
-caption="7cat, resolution 2.5&Aring;" />
-'''THE NADPH BINDING SITE ON BEEF LIVER CATALASE'''<br />
+ {{Structure
+|PDB= 7cat |SIZE=350|CAPTION= <scene name='initialview01'>7cat</scene>, resolution 2.5&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> and <scene name='pdbligand=NDP:NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE'>NDP</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6]
+|GENE=
+}}
+'''THE NADPH BINDING SITE ON BEEF LIVER CATALASE'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-CAT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=NDP:'>NDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CAT OCA].
+CAT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CAT OCA].
 ==Reference==
-The NADPH binding site on beef liver catalase., Fita I, Rossmann MG, Proc Natl Acad Sci U S A. 1985 Mar;82(6):1604-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=3856839 3856839]
+The NADPH binding site on beef liver catalase., Fita I, Rossmann MG, Proc Natl Acad Sci U S A. 1985 Mar;82(6):1604-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3856839 3856839]
 [[Category: Catalase]]
 [[Category: Single protein]]
@@ Line 23: / Line 32: @@
 [[Category: oxidoreductase (h2o2 acceptor)]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:16:57 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:14:20 2008''