6fit: Difference between revisions
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[[Image:6fit.jpg|left|200px]] | [[Image:6fit.jpg|left|200px]] | ||
'''FHIT-TRANSITION STATE ANALOG''' | {{Structure | ||
|PDB= 6fit |SIZE=350|CAPTION= <scene name='initialview01'>6fit</scene>, resolution 2.6Å | |||
|SITE= <scene name='pdbsite=AVE:Active+Site+HIS+Responsible+For+Forming+The+Transient+Nu+...'>AVE</scene> and <scene name='pdbsite=HNE:HIS+Triad+For+Which+This+Family+Was+Named'>HNE</scene> | |||
|LIGAND= <scene name='pdbligand=AMW:ADENOSINE MONOTUNGSTATE'>AMW</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Bis(5'-adenosyl)-triphosphatase Bis(5'-adenosyl)-triphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.29 3.6.1.29] | |||
|GENE= FHIT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |||
}} | |||
'''FHIT-TRANSITION STATE ANALOG''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
6FIT is a [ | 6FIT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FIT OCA]. | ||
==Reference== | ==Reference== | ||
Structure-based analysis of catalysis and substrate definition in the HIT protein family., Lima CD, Klein MG, Hendrickson WA, Science. 1997 Oct 10;278(5336):286-90. PMID:[http:// | Structure-based analysis of catalysis and substrate definition in the HIT protein family., Lima CD, Klein MG, Hendrickson WA, Science. 1997 Oct 10;278(5336):286-90. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9323207 9323207] | ||
[[Category: Bis(5'-adenosyl)-triphosphatase]] | [[Category: Bis(5'-adenosyl)-triphosphatase]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
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[[Category: putative tumor suppressor]] | [[Category: putative tumor suppressor]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:13:26 2008'' | ||
Revision as of 20:13, 20 March 2008
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| , resolution 2.6Å | |||||||
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| Sites: | and | ||||||
| Ligands: | |||||||
| Gene: | FHIT (Homo sapiens) | ||||||
| Activity: | Bis(5'-adenosyl)-triphosphatase, with EC number 3.6.1.29 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
FHIT-TRANSITION STATE ANALOG
OverviewOverview
The histidine triad (HIT) protein family is among the most ubiquitous and highly conserved in nature, but a biological activity has not yet been identified for any member of the HIT family. Fragile histidine triad protein (FHIT) and protein kinase C interacting protein (PKCI) were used in a structure-based approach to elucidate characteristics of in vivo ligands and reactions. Crystallographic structures of apo, substrate analog, pentacovalent transition-state analog, and product states of both enzymes reveal a catalytic mechanism and define substrate characteristics required for catalysis, thus unifying the HIT family as nucleotidyl hydrolases, transferases, or both. The approach described here may be useful in identifying structure-function relations between protein families identified through genomics.
About this StructureAbout this Structure
6FIT is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Structure-based analysis of catalysis and substrate definition in the HIT protein family., Lima CD, Klein MG, Hendrickson WA, Science. 1997 Oct 10;278(5336):286-90. PMID:9323207
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