6cel: Difference between revisions

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Revision as of 20:13, 20 March 2008

File:6cel.gif

, resolution 1.7Å

Sites:

and

Ligands:

and

Gene:

CBH1 (Hypocrea jecorina)

Activity:

Cellulose 1,4-beta-cellobiosidase, with EC number 3.2.1.91

Coordinates:

save as pdb, mmCIF, xml

CBH1 (E212Q) CELLOPENTAOSE COMPLEX

OverviewOverview

Detailed information has been obtained, by means of protein X-ray crystallography, on how a cellulose chain is bound in the cellulose-binding tunnel of cellobiohydrolase I (CBHI), the major cellulase in the hydrolysis of native, crystalline cellulose by the fungus Trichoderma reesei. Three high-resolution crystal structures of different catalytically deficient mutants of CBHI in complex with cellotetraose, cellopentaose and cellohexaose have been refined at 1.9, 1.7 and 1.9 A resolution, respectively. The observed binding of cellooligomers in the tunnel allowed unambiguous identification of ten well-defined subsites for glucosyl units that span a length of approximately 50 A. All bound oligomers have the same directionality and orientation, and the positions of the glucosyl units in each binding site agree remarkably well between the different complexes. The binding mode observed here corresponds to that expected during productive binding of a cellulose chain. The structures support the hypothesis that hydrolysis by CBHI proceeds from the reducing towards the non-reducing end of a cellulose chain, and they provide a structural explanation for the observed distribution of initial hydrolysis products.

About this StructureAbout this Structure

6CEL is a Single protein structure of sequence from Hypocrea jecorina. Full crystallographic information is available from OCA.

ReferenceReference

High-resolution crystal structures reveal how a cellulose chain is bound in the 50 A long tunnel of cellobiohydrolase I from Trichoderma reesei., Divne C, Stahlberg J, Teeri TT, Jones TA, J Mol Biol. 1998 Jan 16;275(2):309-25. PMID:9466911

Page seeded by OCA on Thu Mar 20 19:13:13 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:6cel.gif|left|200px]]<br /><applet load="6cel" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:6cel.gif|left|200px]]
-caption="6cel, resolution 1.7&Aring;" />
-'''CBH1 (E212Q) CELLOPENTAOSE COMPLEX'''<br />
+ {{Structure
+|PDB= 6cel |SIZE=350|CAPTION= <scene name='initialview01'>6cel</scene>, resolution 1.7&Aring;
+|SITE= <scene name='pdbsite=CAT:Catalytic+Site+Including+Mutation+E212q'>CAT</scene> and <scene name='pdbsite=COB:Co-Binding+Site+On+Crystallographic+Dyad.+The+Metal+Ion+...'>COB</scene>
+|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene> and <scene name='pdbligand=CO:COBALT (II) ION'>CO</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase Cellulose 1,4-beta-cellobiosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91]
+|GENE= CBH1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=51453 Hypocrea jecorina])
+}}
+'''CBH1 (E212Q) CELLOPENTAOSE COMPLEX'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-CEL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina] with <scene name='pdbligand=NAG:'>NAG</scene> and <scene name='pdbligand=CO:'>CO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase Cellulose 1,4-beta-cellobiosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] Known structural/functional Sites: <scene name='pdbsite=CAT:Catalytic+Site+Including+Mutation+E212q'>CAT</scene> and <scene name='pdbsite=COB:Co-Binding+Site+On+Crystallographic+Dyad.+The+Metal+Ion+...'>COB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CEL OCA].
+CEL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CEL OCA].
 ==Reference==
-High-resolution crystal structures reveal how a cellulose chain is bound in the 50 A long tunnel of cellobiohydrolase I from Trichoderma reesei., Divne C, Stahlberg J, Teeri TT, Jones TA, J Mol Biol. 1998 Jan 16;275(2):309-25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9466911 9466911]
+High-resolution crystal structures reveal how a cellulose chain is bound in the 50 A long tunnel of cellobiohydrolase I from Trichoderma reesei., Divne C, Stahlberg J, Teeri TT, Jones TA, J Mol Biol. 1998 Jan 16;275(2):309-25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9466911 9466911]
 [[Category: Cellulose 1,4-beta-cellobiosidase]]
 [[Category: Hypocrea jecorina]]
@@ Line 25: / Line 34: @@
 [[Category: hydrolase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:16:17 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:13:13 2008''