Lipase: Difference between revisions
Eric Martz (talk | contribs) No edit summary |
Eric Martz (talk | contribs) No edit summary |
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The carbonyl reforms with the glycerol backbone segment acting as the leaving group (Reaction 2). | The carbonyl reforms with the glycerol backbone segment acting as the leaving group (Reaction 2). | ||
[[Image:M0218. | [[Image:M0218.stg02r.gif|center|]] | ||
A water molecule then donates a proton to the histidine, creating a reactive hydroxyl anion. The hydroxyl anion can then attack the carbonyl carbon of the lipid, forming another negatively charged tetrahedral intermediate which is stabilized in the oxyanion hole (Reaction 3). | A water molecule then donates a proton to the histidine, creating a reactive hydroxyl anion. The hydroxyl anion can then attack the carbonyl carbon of the lipid, forming another negatively charged tetrahedral intermediate which is stabilized in the oxyanion hole (Reaction 3). | ||