PLC beta 3 Gq: Difference between revisions

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Shir Navot, Michal Harel, Joel L. Sussman

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 <scene name='70/701452/Fig7/1'>An extended loop</scene> (residues 254-268) between EF hands 3 and 4 of PLC- β3 interacts with the GTP-binding region of Gαq. Asn260 of the EF3/4 loop promotes GTP hydrolysis by interaction with the side chain of Gln209 of Gαq, which rearranges during GTP hydrolysis to stabilize the transition state mimicked by GDP•AlF4–•H20. Asn260 also interacts with Glu212 to stabilize switch 1 for GTP hydrolysis.
-Other effectors are known to engage residues (pink) in the <scene name='70/701452/Fig3/3'>effector-binding site</scene> within Gα subunits. There are a large family of regulator of G protein signaling (RGS) proteins that independently accelerates the GTP hydrolysis in the <scene name='70/701452/Fig4/3'>GTPase domain</scene> composed out of several residues (brown). In comparison to PLC-β3 interacts with residues (blue) on Gαq that <scene name='70/701452/Fig5/4'>overlaps almost completely</scene> with portions of Gα subunits needed for engagement of RGS proteins and the effector-binding region.
+Other effectors are known to engage residues (pink) in the <scene name='70/701452/Fig3/3'>effector-binding site</scene> <ref>PMID:20966218</ref> within Gα subunits. There are a large family of regulator of G protein signaling (RGS) proteins that independently accelerates the GTP hydrolysis in the <scene name='70/701452/Fig4/3'>GTPase domain</scene> composed out of several residues (brown). In comparison to PLC-β3 interacts with residues (blue) on Gαq that <scene name='70/701452/Fig5/4'>overlaps almost completely</scene> with portions of Gα subunits needed for engagement of RGS proteins and the effector-binding region.
 '''Critical residues in the interface:'''