437d: Difference between revisions

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CRYSTAL STRUCTURE OF AN RNA PSEUDOKNOT FROM BEET WESTERN YELLOW VIRUS INVOLVED IN RIBOSOMAL FRAMESHIFTING

OverviewOverview

Many viruses regulate translation of polycistronic mRNA using a -1 ribosomal frameshift induced by an RNA pseudoknot. A pseudoknot has two stems that form a quasi-continuous helix and two connecting loops. A 1.6 A crystal structure of the beet western yellow virus (BWYV) pseudoknot reveals rotation and a bend at the junction of the two stems. A loop base is inserted in the major groove of one stem with quadruple-base interactions. The second loop forms a new minor-groove triplex motif with the other stem, involving 2'-OH and triple-base interactions, as well as sodium ion coordination. Overall, the number of hydrogen bonds stabilizing the tertiary interactions exceeds the number involved in Watson-Crick base pairs. This structure will aid mechanistic analyses of ribosomal frameshifting.

About this StructureAbout this Structure

437D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

ReferenceReference

Minor groove RNA triplex in the crystal structure of a ribosomal frameshifting viral pseudoknot., Su L, Chen L, Egli M, Berger JM, Rich A, Nat Struct Biol. 1999 Mar;6(3):285-92. PMID:10074948

Page seeded by OCA on Thu Mar 20 19:08:15 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:437d.gif|left|200px]]<br /><applet load="437d" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:437d.gif|left|200px]]
-caption="437d, resolution 1.6&Aring;" />
-'''CRYSTAL STRUCTURE OF AN RNA PSEUDOKNOT FROM BEET WESTERN YELLOW VIRUS INVOLVED IN RIBOSOMAL FRAMESHIFTING'''<br />
+ {{Structure
+|PDB= 437d |SIZE=350|CAPTION= <scene name='initialview01'>437d</scene>, resolution 1.6&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=NA:SODIUM ION'>NA</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''CRYSTAL STRUCTURE OF AN RNA PSEUDOKNOT FROM BEET WESTERN YELLOW VIRUS INVOLVED IN RIBOSOMAL FRAMESHIFTING'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=437D OCA].
+D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=437D OCA].
 ==Reference==
-Minor groove RNA triplex in the crystal structure of a ribosomal frameshifting viral pseudoknot., Su L, Chen L, Egli M, Berger JM, Rich A, Nat Struct Biol. 1999 Mar;6(3):285-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10074948 10074948]
+Minor groove RNA triplex in the crystal structure of a ribosomal frameshifting viral pseudoknot., Su L, Chen L, Egli M, Berger JM, Rich A, Nat Struct Biol. 1999 Mar;6(3):285-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10074948 10074948]
 [[Category: Protein complex]]
 [[Category: Berger, J M.]]
@@ Line 24: / Line 33: @@
 [[Category: viral frameshifting]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:12:11 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:08:15 2008''