4xgz: Difference between revisions
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''' | ==Crystal structure of human paxillin LD2 motif in complex with Fab fragment== | ||
<StructureSection load='4xgz' size='340' side='right' caption='[[4xgz]], [[Resolution|resolution]] 2.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4xgz]] is a 36 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XGZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XGZ FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4xh2|4xh2]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xgz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xgz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xgz RCSB], [http://www.ebi.ac.uk/pdbsum/4xgz PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Focal adhesion protein paxillin links integrin and growth factor signaling to actin cytoskeleton. Most of paxillin signaling activity is regulated via leucine-rich LD motifs (LD1-LD5) located at the N-terminus. Here, we demonstrate a method to engineer highly selective synthetic antibodies (sABs) against LD2 and LD4 which are binding sites for focal adhesion kinase (FAK) and other proteins. Phage display selections against peptides were used to generate sABs recognizing each LD motif. In the obtained X-ray crystal structures of the LD-sAB complexes the LD motifs are helical and bind sABs through a hydrophobic side, similarly as in the structures with natural paxillin partners. The sABs are capable of pulling down endogenous paxillin in complex with FAK and can visualize paxillin in focal adhesions in cells. They were also used as selective inhibitors to effectively compete with focal adhesion targeting domain of FAK for the binding to LD2 and LD4. The sABs are tools for investigation of paxillin LD binding "platforms" and are capable of inhibiting paxillin interactions; and thereby useful as potential therapeutics in the future. | |||
Engineering synthetic antibody inhibitors specific for LD2 or LD4 motifs of paxillin.,Nocula-Lugowska M, Lugowski M, Salgia R, Kossiakoff AA J Mol Biol. 2015 Jun 15. pii: S0022-2836(15)00344-7. doi:, 10.1016/j.jmb.2015.06.004. PMID:26087144<ref>PMID:26087144</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Kossiakoff, A A]] | |||
[[Category: Lugowski, M]] | |||
[[Category: Nocula-Lugowska, M]] | |||
[[Category: Salgia, R]] | [[Category: Salgia, R]] | ||
[[Category: | [[Category: Cell adhesion]] | ||
[[Category: | [[Category: Complex]] | ||
[[Category: Fab fragment]] | |||
[[Category: Focal adhesion]] | |||
[[Category: Immunoglobulin]] | |||
[[Category: Ld motif]] | |||
[[Category: Paxillin]] | |||
[[Category: Synthetic antibody]] | |||
Revision as of 15:12, 1 July 2015
Crystal structure of human paxillin LD2 motif in complex with Fab fragmentCrystal structure of human paxillin LD2 motif in complex with Fab fragment
Structural highlights
Publication Abstract from PubMedFocal adhesion protein paxillin links integrin and growth factor signaling to actin cytoskeleton. Most of paxillin signaling activity is regulated via leucine-rich LD motifs (LD1-LD5) located at the N-terminus. Here, we demonstrate a method to engineer highly selective synthetic antibodies (sABs) against LD2 and LD4 which are binding sites for focal adhesion kinase (FAK) and other proteins. Phage display selections against peptides were used to generate sABs recognizing each LD motif. In the obtained X-ray crystal structures of the LD-sAB complexes the LD motifs are helical and bind sABs through a hydrophobic side, similarly as in the structures with natural paxillin partners. The sABs are capable of pulling down endogenous paxillin in complex with FAK and can visualize paxillin in focal adhesions in cells. They were also used as selective inhibitors to effectively compete with focal adhesion targeting domain of FAK for the binding to LD2 and LD4. The sABs are tools for investigation of paxillin LD binding "platforms" and are capable of inhibiting paxillin interactions; and thereby useful as potential therapeutics in the future. Engineering synthetic antibody inhibitors specific for LD2 or LD4 motifs of paxillin.,Nocula-Lugowska M, Lugowski M, Salgia R, Kossiakoff AA J Mol Biol. 2015 Jun 15. pii: S0022-2836(15)00344-7. doi:, 10.1016/j.jmb.2015.06.004. PMID:26087144[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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