3bhf: Difference between revisions
New page: left|200px<br /><applet load="3bhf" size="350" color="white" frame="true" align="right" spinBox="true" caption="3bhf, resolution 2.10Å" /> '''Crystal structure of... |
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[[Image:3bhf.jpg|left|200px]] | [[Image:3bhf.jpg|left|200px]] | ||
'''Crystal structure of R49K mutant of Monomeric Sarcosine Oxidase crystallized in PEG as precipitant''' | {{Structure | ||
|PDB= 3bhf |SIZE=350|CAPTION= <scene name='initialview01'>3bhf</scene>, resolution 2.10Å | |||
|SITE= <scene name='pdbsite=AC1:Cl+Binding+Site+For+Residue+A+401'>AC1</scene>, <scene name='pdbsite=AC2:Cl+Binding+Site+For+Residue+B+401'>AC2</scene>, <scene name='pdbsite=AC3:Fad+Binding+Site+For+Residue+A+400'>AC3</scene> and <scene name='pdbsite=AC4:Fad+Binding+Site+For+Residue+B+400'>AC4</scene> | |||
|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=FAD:FLAVIN-ADENINE DINUCLEOTIDE'>FAD</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Sarcosine_oxidase Sarcosine oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.3.1 1.5.3.1] | |||
|GENE= soxA, sox ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1409 Bacillus sp.]) | |||
}} | |||
'''Crystal structure of R49K mutant of Monomeric Sarcosine Oxidase crystallized in PEG as precipitant''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
3BHF is a [ | 3BHF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_sp. Bacillus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BHF OCA]. | ||
==Reference== | ==Reference== | ||
Arginine 49 Is a Bifunctional Residue Important in Catalysis and Biosynthesis of Monomeric Sarcosine Oxidase: A Context-Sensitive Model for the Electrostatic Impact of Arginine to Lysine Mutations(,)., Hassan-Abdallah A, Zhao G, Chen ZW, Mathews FS, Schuman Jorns M, Biochemistry. 2008 Feb 6;. PMID:[http:// | Arginine 49 Is a Bifunctional Residue Important in Catalysis and Biosynthesis of Monomeric Sarcosine Oxidase: A Context-Sensitive Model for the Electrostatic Impact of Arginine to Lysine Mutations(,)., Hassan-Abdallah A, Zhao G, Chen ZW, Mathews FS, Schuman Jorns M, Biochemistry. 2008 Feb 6;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18251505 18251505] | ||
[[Category: Bacillus sp.]] | [[Category: Bacillus sp.]] | ||
[[Category: Sarcosine oxidase]] | [[Category: Sarcosine oxidase]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:58:55 2008'' | ||
Revision as of 19:58, 20 March 2008
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| , resolution 2.10Å | |||||||
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| Sites: | , , and | ||||||
| Ligands: | and | ||||||
| Gene: | soxA, sox (Bacillus sp.) | ||||||
| Activity: | Sarcosine oxidase, with EC number 1.5.3.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of R49K mutant of Monomeric Sarcosine Oxidase crystallized in PEG as precipitant
OverviewOverview
Monomeric sarcosine oxidase (MSOX) contains covalently bound FAD and catalyzes the oxidative demethylation of sarcosine ( N-methylglycine). The side chain of Arg49 is in van der Waals contact with the si face of the flavin ring; sarcosine binds just above the re face. Covalent flavin attachment requires a basic residue (Arg or Lys) at position 49. Although flavinylation is scarcely affected, mutation of Arg49 to Lys causes a 40-fold decrease in k cat and a 150-fold decrease in k cat/ K m sarcosine. The overall structure of the Arg49Lys mutant is very similar to wild-type MSOX; the side chain of Lys49 in the mutant is nearly congruent to that of Arg49 in the wild-type enzyme. The Arg49Lys mutant exhibits several features consistent with a less electropositive active site: (1) Charge transfer bands observed for mutant enzyme complexes with competitive inhibitors absorb at higher energy than the corresponding wild-type complexes. (2) The p K a for ionization at N(3)H of FAD is more than two pH units higher in the mutant than in wild-type MSOX. (3) The reduction potential of the oxidized/radical couple in the mutant is 100 mV lower than in the wild-type enzyme. The lower reduction potential is likely to be a major cause of the reduced catalytic activity of the mutant. Electrostatic interactions with Arg49 play an important role in catalysis and covalent flavinylation. A context-sensitive model for the electrostatic impact of an arginine to lysine mutation can account for the dramatically different consequences of the Arg49Lys mutation on MSOX catalysis and holoenzyme biosysnthesis.
About this StructureAbout this Structure
3BHF is a Single protein structure of sequence from Bacillus sp.. Full crystallographic information is available from OCA.
ReferenceReference
Arginine 49 Is a Bifunctional Residue Important in Catalysis and Biosynthesis of Monomeric Sarcosine Oxidase: A Context-Sensitive Model for the Electrostatic Impact of Arginine to Lysine Mutations(,)., Hassan-Abdallah A, Zhao G, Chen ZW, Mathews FS, Schuman Jorns M, Biochemistry. 2008 Feb 6;. PMID:18251505
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