3bgs: Difference between revisions

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Revision as of 19:58, 20 March 2008

File:3bgs.jpg

, resolution 2.099Å

Sites:

, , and

Ligands:

and

Gene:

NP, PNP (Homo sapiens)

Activity:

Purine-nucleoside phosphorylase, with EC number 2.4.2.1

Coordinates:

save as pdb, mmCIF, xml

Structure of human purine nucleoside phosphorylase with L-DADMe-ImmH and phosphate

OverviewOverview

Human purine nucleoside phosphorylase (PNP) was crystallized with transition-state analogue inhibitors Immucillin-H and DADMe-Immucillin-H synthesized with ribosyl mimics of l-stereochemistry. The inhibitors demonstrate that major driving forces for tight binding of these analogues are the leaving group interaction and the cationic mimicry of the transition state, even though large geometric changes occur with d-Immucillins and l-Immucillins bound to human PNP.

About this StructureAbout this Structure

3BGS is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

L-Enantiomers of transition state analogue inhibitors bound to human purine nucleoside phosphorylase., Rinaldo-Matthis A, Murkin AS, Ramagopal UA, Clinch K, Mee SP, Evans GB, Tyler PC, Furneaux RH, Almo SC, Schramm VL, J Am Chem Soc. 2008 Jan 23;130(3):842-4. Epub 2007 Dec 23. PMID:18154341

Page seeded by OCA on Thu Mar 20 18:58:47 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:3bgs.jpg|left|200px]]<br /><applet load="3bgs" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:3bgs.jpg|left|200px]]
-caption="3bgs, resolution 2.099&Aring;" />
-'''Structure of human purine nucleoside phosphorylase with L-DADMe-ImmH and phosphate'''<br />
+ {{Structure
+|PDB= 3bgs |SIZE=350|CAPTION= <scene name='initialview01'>3bgs</scene>, resolution 2.099&Aring;
+|SITE= <scene name='pdbsite=AC1:Po4+Binding+Site+For+Residue+A+302'>AC1</scene>, <scene name='pdbsite=AC2:Po4+Binding+Site+For+Residue+A+303'>AC2</scene>, <scene name='pdbsite=AC3:Po4+Binding+Site+For+Residue+A+304'>AC3</scene> and <scene name='pdbsite=AC4:Dih+Binding+Site+For+Residue+A+301'>AC4</scene>
+|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=DIH:3-HYDROXY-4-HYDROXYMETHYL-1-(4-OXO-4,4A,5,7A-TETRAHYDRO-3H-PYRROLO[3,2-D]PYRIMIDIN-7-YLMETHYL)-PYRROLIDINIUM'>DIH</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Purine-nucleoside_phosphorylase Purine-nucleoside phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.1 2.4.2.1]
+|GENE= NP, PNP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+}}
+'''Structure of human purine nucleoside phosphorylase with L-DADMe-ImmH and phosphate'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-BGS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=DIH:'>DIH</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Purine-nucleoside_phosphorylase Purine-nucleoside phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.1 2.4.2.1] Known structural/functional Sites: <scene name='pdbsite=AC1:Po4+Binding+Site+For+Residue+A+302'>AC1</scene>, <scene name='pdbsite=AC2:Po4+Binding+Site+For+Residue+A+303'>AC2</scene>, <scene name='pdbsite=AC3:Po4+Binding+Site+For+Residue+A+304'>AC3</scene> and <scene name='pdbsite=AC4:Dih+Binding+Site+For+Residue+A+301'>AC4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BGS OCA].
+BGS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BGS OCA].
 ==Reference==
-L-Enantiomers of transition state analogue inhibitors bound to human purine nucleoside phosphorylase., Rinaldo-Matthis A, Murkin AS, Ramagopal UA, Clinch K, Mee SP, Evans GB, Tyler PC, Furneaux RH, Almo SC, Schramm VL, J Am Chem Soc. 2008 Jan 23;130(3):842-4. Epub 2007 Dec 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18154341 18154341]
+L-Enantiomers of transition state analogue inhibitors bound to human purine nucleoside phosphorylase., Rinaldo-Matthis A, Murkin AS, Ramagopal UA, Clinch K, Mee SP, Evans GB, Tyler PC, Furneaux RH, Almo SC, Schramm VL, J Am Chem Soc. 2008 Jan 23;130(3):842-4. Epub 2007 Dec 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18154341 18154341]
 [[Category: Homo sapiens]]
 [[Category: Purine-nucleoside phosphorylase]]
@@ Line 25: / Line 34: @@
 [[Category: transition state analogue]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 27 07:48:50 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:58:47 2008''