3b95: Difference between revisions
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'''EuHMT1 (Glp) Ankyrin Repeat Domain (Structure 2)''' | {{Structure | ||
|PDB= 3b95 |SIZE=350|CAPTION= <scene name='initialview01'>3b95</scene>, resolution 2.99Å | |||
|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+B+1'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+B+3'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+B+4'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+A+5'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+A+6'>AC5</scene>, <scene name='pdbsite=AC6:So4+Binding+Site+For+Residue+A+7'>AC6</scene>, <scene name='pdbsite=AC7:So4+Binding+Site+For+Residue+A+8'>AC7</scene>, <scene name='pdbsite=AC8:So4+Binding+Site+For+Residue+B+9'>AC8</scene> and <scene name='pdbsite=AC9:So4+Binding+Site+For+Residue+A+10'>AC9</scene> | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | |||
|ACTIVITY= | |||
|GENE= EHMT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |||
}} | |||
'''EuHMT1 (Glp) Ankyrin Repeat Domain (Structure 2)''' | |||
==Overview== | |||
Histone modifications have important roles in transcriptional control, mitosis and heterochromatin formation. G9a and G9a-like protein (GLP) are euchromatin-associated methyltransferases that repress transcription by mono- and dimethylating histone H3 at Lys9 (H3K9). Here we demonstrate that the ankyrin repeat domains of G9a and GLP bind with strong preference to N-terminal H3 peptides containing mono- or dimethyl K9. X-ray crystallography revealed the basis for recognition of the methylated lysine by a partial hydrophobic cage with three tryptophans and one acidic residue. Substitution of key residues in the cage eliminated the H3 tail interaction. Hence, G9a and GLP contain a new type of methyllysine binding module (the ankyrin repeat domains) and are the first examples of protein (histone) methyltransferases harboring in a single polypeptide the activities that generate and read the same epigenetic mark. | |||
==About this Structure== | ==About this Structure== | ||
3B95 is a [ | 3B95 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B95 OCA]. | ||
==Reference== | |||
The ankyrin repeats of G9a and GLP histone methyltransferases are mono- and dimethyllysine binding modules., Collins RE, Northrop JP, Horton JR, Lee DY, Zhang X, Stallcup MR, Cheng X, Nat Struct Mol Biol. 2008 Mar;15(3):245-50. Epub 2008 Feb 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18264113 18264113] | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| Line 17: | Line 32: | ||
[[Category: transferase/structual protein complex]] | [[Category: transferase/structual protein complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:57:14 2008'' | ||
Revision as of 19:57, 20 March 2008
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| , resolution 2.99Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , , , , , , and | ||||||
| Ligands: | |||||||
| Gene: | EHMT1 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
EuHMT1 (Glp) Ankyrin Repeat Domain (Structure 2)
OverviewOverview
Histone modifications have important roles in transcriptional control, mitosis and heterochromatin formation. G9a and G9a-like protein (GLP) are euchromatin-associated methyltransferases that repress transcription by mono- and dimethylating histone H3 at Lys9 (H3K9). Here we demonstrate that the ankyrin repeat domains of G9a and GLP bind with strong preference to N-terminal H3 peptides containing mono- or dimethyl K9. X-ray crystallography revealed the basis for recognition of the methylated lysine by a partial hydrophobic cage with three tryptophans and one acidic residue. Substitution of key residues in the cage eliminated the H3 tail interaction. Hence, G9a and GLP contain a new type of methyllysine binding module (the ankyrin repeat domains) and are the first examples of protein (histone) methyltransferases harboring in a single polypeptide the activities that generate and read the same epigenetic mark.
About this StructureAbout this Structure
3B95 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
The ankyrin repeats of G9a and GLP histone methyltransferases are mono- and dimethyllysine binding modules., Collins RE, Northrop JP, Horton JR, Lee DY, Zhang X, Stallcup MR, Cheng X, Nat Struct Mol Biol. 2008 Mar;15(3):245-50. Epub 2008 Feb 10. PMID:18264113
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