PLC beta 3 Gq: Difference between revisions
Shir Navot (talk | contribs) No edit summary |
Shir Navot (talk | contribs) No edit summary |
||
| Line 5: | Line 5: | ||
== Structural highlights == | == Structural highlights == | ||
The Gαq subunit consists two domains, one is the GTPase domain and the other is the alpha helical domain. These domains include three regions called switch regions I-III. | The Gαq subunit consists two domains, one is the GTPase domain and the other is the alpha helical domain. These domains include three regions called switch regions I-III. | ||
<scene name='70/701452/Fig1/9'>The PLC- β3 has several domains</scene>consisting of N-terminal PH domain, a series of four EF hands, a catalytic TIM barrel and a C2 domain. | <scene name='70/701452/Fig1/9'>The PLC- β3 has several domains</scene> consisting of N-terminal PH domain, a series of four EF hands, a catalytic TIM barrel and a C2 domain. | ||
PLC- β3 engages Gαq throughout three regions. First, an extended loop between the third and fourth EF hands of PLC- β3 directly buttresses switch residues critical for GTP hydrolysis by Gαq. Second, the region of PLC- β3 that connects the catalytic TIM barrel and the C2 domain interacts with both switches 1 and 2 of Gαq. Third, a segment composed of a helix-turn-helix at the C terminus of the C2 domain resides primarily within a shallow declivity on the surface of Gαq formed by switch 2 and α3. | PLC- β3 engages Gαq throughout three regions. First, an extended loop between the third and fourth EF hands of PLC- β3 directly buttresses switch residues critical for GTP hydrolysis by Gαq. Second, the region of PLC- β3 that connects the catalytic TIM barrel and the C2 domain interacts with both switches 1 and 2 of Gαq. Third, a segment composed of a helix-turn-helix at the C terminus of the C2 domain resides primarily within a shallow declivity on the surface of Gαq formed by switch 2 and α3. | ||