2vf2: Difference between revisions

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Revision as of 19:45, 20 March 2008

File:2vf2.jpg

, resolution 2.35Å

Sites:

, , , and

Ligands:

and

Activity:

2,6-dioxo-6-phenylhexa-3-enoate hydrolase, with EC number 3.7.1.8

Coordinates:

save as pdb, mmCIF, xml

X-RAY CRYSTAL STRUCTURE OF HSAD FROM MYCOBACTERIUM TUBERCULOSIS

OverviewOverview

Tuberculosis is a major cause of death worldwide. Understanding of the pathogenicity of Mycobacterium tuberculosis has been advanced by gene analysis and has led to the identification of genes that are important for intracellular survival in macrophages. One of these genes encodes HsaD, a meta-cleavage product (MCP) hydrolase that catalyzes the hydrolytic cleavage of a carbon-carbon bond in cholesterol metabolism. This paper describes the production of HsaD as a recombinant protein and, following crystallization, the determination of its three-dimensional structure to 2.35 A resolution by X-ray crystallography at the Diamond Light Source in Oxfordshire, England. To the authors' knowledge, this study constitutes the first report of a structure determined at the new synchrotron facility. The volume of the active-site cleft of the HsaD enzyme is more than double the corresponding active-site volumes of related MCP hydrolases involved in the catabolism of aromatic compounds, consistent with the specificity of HsaD for steroids such as cholesterol. Knowledge of the structure of the enzyme facilitates the design of inhibitors.

About this StructureAbout this Structure

2VF2 is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

ReferenceReference

Structure of HsaD, a steroid-degrading hydrolase, from Mycobacterium tuberculosis., Lack N, Lowe ED, Liu J, Eltis LD, Noble ME, Sim E, Westwood IM, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jan 1;64(Pt 1):2-7., Epub 2007 Dec 20. PMID:18097091

Page seeded by OCA on Thu Mar 20 18:45:54 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2vf2.jpg|left|200px]]<br /><applet load="2vf2" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2vf2.jpg|left|200px]]
-caption="2vf2, resolution 2.35&Aring;" />
-'''X-RAY CRYSTAL STRUCTURE OF HSAD FROM MYCOBACTERIUM TUBERCULOSIS'''<br />
+ {{Structure
+|PDB= 2vf2 |SIZE=350|CAPTION= <scene name='initialview01'>2vf2</scene>, resolution 2.35&Aring;
+|SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+B'>AC1</scene>, <scene name='pdbsite=AC2:Gol+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Gol+Binding+Site+For+Chain+B'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Chain+A'>AC4</scene> and <scene name='pdbsite=AC5:So4+Binding+Site+For+Chain+B'>AC5</scene>
+|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/2,6-dioxo-6-phenylhexa-3-enoate_hydrolase 2,6-dioxo-6-phenylhexa-3-enoate hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.8 3.7.1.8]
+|GENE=
+}}
+'''X-RAY CRYSTAL STRUCTURE OF HSAD FROM MYCOBACTERIUM TUBERCULOSIS'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-VF2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/2,6-dioxo-6-phenylhexa-3-enoate_hydrolase 2,6-dioxo-6-phenylhexa-3-enoate hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.8 3.7.1.8] Known structural/functional Sites: <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+B'>AC1</scene>, <scene name='pdbsite=AC2:Gol+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Gol+Binding+Site+For+Chain+B'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Chain+A'>AC4</scene> and <scene name='pdbsite=AC5:So4+Binding+Site+For+Chain+B'>AC5</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VF2 OCA].
+VF2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VF2 OCA].
 ==Reference==
-Structure of HsaD, a steroid-degrading hydrolase, from Mycobacterium tuberculosis., Lack N, Lowe ED, Liu J, Eltis LD, Noble ME, Sim E, Westwood IM, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jan 1;64(Pt 1):2-7., Epub 2007 Dec 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18097091 18097091]
+Structure of HsaD, a steroid-degrading hydrolase, from Mycobacterium tuberculosis., Lack N, Lowe ED, Liu J, Eltis LD, Noble ME, Sim E, Westwood IM, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jan 1;64(Pt 1):2-7., Epub 2007 Dec 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18097091 18097091]
 [[Category: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase]]
 [[Category: Mycobacterium tuberculosis]]
@@ Line 28: / Line 37: @@
 [[Category: serine hydrolase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:55:27 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:45:54 2008''