2rii: Difference between revisions

← Older edit Newer edit →

Revision as of 19:36, 20 March 2008

File:2rii.jpg

, resolution 2.60Å

Ligands:

Gene:

PRDX1, PAGA, PAGB, TDPX2 (Homo sapiens), SRXN1, C20orf139, SRX (Homo sapiens)

Activity:

Peroxiredoxin, with EC number 1.11.1.15

Coordinates:

save as pdb, mmCIF, xml

Crystal Structure of Human Peroxiredoxin I in complex with Sulfiredoxin

OverviewOverview

Typical 2-Cys peroxiredoxins (Prxs) have an important role in regulating hydrogen peroxide-mediated cell signalling. In this process, Prxs can become inactivated through the hyperoxidation of an active site Cys residue to Cys sulphinic acid. The unique repair of this moiety by sulphiredoxin (Srx) restores peroxidase activity and terminates the signal. The hyperoxidized form of Prx exists as a stable decameric structure with each active site buried. Therefore, it is unclear how Srx can access the sulphinic acid moiety. Here we present the 2.6 A crystal structure of the human Srx-PrxI complex. This complex reveals the complete unfolding of the carboxy terminus of Prx, and its unexpected packing onto the backside of Srx away from the Srx active site. Binding studies and activity analyses of site-directed mutants at this interface show that the interaction is required for repair to occur. Moreover, rearrangements in the Prx active site lead to a juxtaposition of the Prx Gly-Gly-Leu-Gly and Srx ATP-binding motifs, providing a structural basis for the first step of the catalytic mechanism. The results also suggest that the observed interactions may represent a common mode for other proteins to bind to Prxs.

About this StructureAbout this Structure

2RII is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the sulphiredoxin-peroxiredoxin complex reveals an essential repair embrace., Jonsson TJ, Johnson LC, Lowther WT, Nature. 2008 Jan 3;451(7174):98-101. PMID:18172504

Page seeded by OCA on Thu Mar 20 18:36:25 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2rii.jpg|left|200px]]<br /><applet load="2rii" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2rii.jpg|left|200px]]
-caption="2rii, resolution 2.60&Aring;" />
-'''Crystal Structure of Human Peroxiredoxin I in complex with Sulfiredoxin'''<br />
+ {{Structure
+|PDB= 2rii |SIZE=350|CAPTION= <scene name='initialview01'>2rii</scene>, resolution 2.60&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Peroxiredoxin Peroxiredoxin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.15 1.11.1.15]
+|GENE= PRDX1, PAGA, PAGB, TDPX2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), SRXN1, C20orf139, SRX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+}}
+'''Crystal Structure of Human Peroxiredoxin I in complex with Sulfiredoxin'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-RII is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Peroxiredoxin Peroxiredoxin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.15 1.11.1.15] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RII OCA].
+RII is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RII OCA].
 ==Reference==
-Structure of the sulphiredoxin-peroxiredoxin complex reveals an essential repair embrace., Jonsson TJ, Johnson LC, Lowther WT, Nature. 2008 Jan 3;451(7174):98-101. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18172504 18172504]
+Structure of the sulphiredoxin-peroxiredoxin complex reveals an essential repair embrace., Jonsson TJ, Johnson LC, Lowther WT, Nature. 2008 Jan 3;451(7174):98-101. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18172504 18172504]
 [[Category: Homo sapiens]]
 [[Category: Peroxiredoxin]]
@@ Line 33: / Line 42: @@
 [[Category: sulfinic acid reductase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar  2 11:11:11 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:36:25 2008''