2raj: Difference between revisions

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Revision as of 19:34, 20 March 2008

File:2raj.jpg

, resolution 2.45Å

Ligands:

Gene:

SNX9, SH3PX1, SH3PXD3A (Homo sapiens)

Coordinates:

save as pdb, mmCIF, xml

SO4 bound PX-BAR membrane remodeling unit of Sorting Nexin 9

OverviewOverview

Sorting nexins (SNXs) form a family of proteins known to interact with components in the endosomal system and to regulate various steps of vesicle transport. Sorting nexin 9 (SNX9) is involved in the late stages of clathrin-mediated endocytosis in non-neuronal cells, where together with the GTPase dynamin, it participates in the formation and scission of the vesicle neck. We report here crystal structures of the functional membrane-remodeling unit of SNX9 and show that it efficiently tubulates lipid membranes in vivo and in vitro. Elucidation of the protein superdomain structure, together with mutational analysis and biochemical and cell biological experiments, demonstrated how the SNX9 PX and BAR domains work in concert in targeting and tubulation of phosphoinositide-containing membranes. The study provides insights into the SNX9-induced membrane modulation mechanism.

About this StructureAbout this Structure

2RAJ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

The PX-BAR membrane-remodeling unit of sorting nexin 9., Pylypenko O, Lundmark R, Rasmuson E, Carlsson SR, Rak A, EMBO J. 2007 Nov 14;26(22):4788-800. Epub 2007 Oct 18. PMID:17948057

Page seeded by OCA on Thu Mar 20 18:34:09 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2raj.jpg|left|200px]]<br /><applet load="2raj" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2raj.jpg|left|200px]]
-caption="2raj, resolution 2.45&Aring;" />
-'''SO4 bound PX-BAR membrane remodeling unit of Sorting Nexin 9'''<br />
+ {{Structure
+|PDB= 2raj |SIZE=350|CAPTION= <scene name='initialview01'>2raj</scene>, resolution 2.45&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
+|ACTIVITY=
+|GENE= SNX9, SH3PX1, SH3PXD3A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+}}
+'''SO4 bound PX-BAR membrane remodeling unit of Sorting Nexin 9'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-RAJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAJ OCA].
+RAJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAJ OCA].
 ==Reference==
-The PX-BAR membrane-remodeling unit of sorting nexin 9., Pylypenko O, Lundmark R, Rasmuson E, Carlsson SR, Rak A, EMBO J. 2007 Nov 14;26(22):4788-800. Epub 2007 Oct 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17948057 17948057]
+The PX-BAR membrane-remodeling unit of sorting nexin 9., Pylypenko O, Lundmark R, Rasmuson E, Carlsson SR, Rak A, EMBO J. 2007 Nov 14;26(22):4788-800. Epub 2007 Oct 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17948057 17948057]
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
@@ Line 26: / Line 35: @@
 [[Category: tubulation]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:45:56 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:34:09 2008''