2r6g: Difference between revisions

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Revision as of 19:33, 20 March 2008

File:2r6g.jpg

, resolution 2.80Å

Ligands:

and

Gene:

malK (Escherichia coli), malE (Escherichia coli), malF (Escherichia coli), malG (Escherichia coli)

Activity:

Maltose-transporting ATPase, with EC number 3.6.3.19

Coordinates:

save as pdb, mmCIF, xml

The Crystal Structure of the E. coli Maltose Transporter

OverviewOverview

The maltose uptake system of Escherichia coli is a well-characterized member of the ATP-binding cassette transporter superfamily. Here we present the 2.8-A crystal structure of the intact maltose transporter in complex with the maltose-binding protein, maltose and ATP. This structure, stabilized by a mutation that prevents ATP hydrolysis, captures the ATP-binding cassette dimer in a closed, ATP-bound conformation. Maltose is occluded within a solvent-filled cavity at the interface of the two transmembrane subunits, about halfway into the lipid bilayer. The binding protein docks onto the entrance of the cavity in an open conformation and serves as a cap to ensure unidirectional translocation of the sugar molecule. These results provide direct evidence for a concerted mechanism of transport in which solute is transferred from the binding protein to the transmembrane subunits when the cassette dimer closes to hydrolyse ATP.

About this StructureAbout this Structure

2R6G is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of a catalytic intermediate of the maltose transporter., Oldham ML, Khare D, Quiocho FA, Davidson AL, Chen J, Nature. 2007 Nov 22;450(7169):515-21. PMID:18033289

Page seeded by OCA on Thu Mar 20 18:33:05 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2r6g.jpg|left|200px]]<br /><applet load="2r6g" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2r6g.jpg|left|200px]]
-caption="2r6g, resolution 2.80&Aring;" />
-'''The Crystal Structure of the E. coli Maltose Transporter'''<br />
+ {{Structure
+|PDB= 2r6g |SIZE=350|CAPTION= <scene name='initialview01'>2r6g</scene>, resolution 2.80&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=MAL:MALTOSE'>MAL</scene> and <scene name='pdbligand=ATP:ADENOSINE-5'-TRIPHOSPHATE'>ATP</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Maltose-transporting_ATPase Maltose-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.19 3.6.3.19]
+|GENE= malK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), malE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), malF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), malG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+}}
+'''The Crystal Structure of the E. coli Maltose Transporter'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-R6G is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MAL:'>MAL</scene> and <scene name='pdbligand=ATP:'>ATP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Maltose-transporting_ATPase Maltose-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.19 3.6.3.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R6G OCA].
+R6G is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R6G OCA].
 ==Reference==
-Crystal structure of a catalytic intermediate of the maltose transporter., Oldham ML, Khare D, Quiocho FA, Davidson AL, Chen J, Nature. 2007 Nov 22;450(7169):515-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18033289 18033289]
+Crystal structure of a catalytic intermediate of the maltose transporter., Oldham ML, Khare D, Quiocho FA, Davidson AL, Chen J, Nature. 2007 Nov 22;450(7169):515-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18033289 18033289]
 [[Category: Escherichia coli]]
 [[Category: Maltose-transporting ATPase]]
@@ Line 38: / Line 47: @@
 [[Category: transmembrane]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:44:57 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:33:05 2008''