2r6e: Difference between revisions

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Revision as of 19:33, 20 March 2008

File:2r6e.jpg

, resolution 5.019Å

Ligands:

Gene:

DnaB (Geobacillus stearothermophilus)

Coordinates:

save as pdb, mmCIF, xml

Crystal Form B2

OverviewOverview

The complex between the DnaB helicase and the DnaG primase unwinds duplex DNA at the eubacterial replication fork and synthesizes the Okazaki RNA primers. The crystal structures of hexameric DnaB and its complex with the helicase binding domain (HBD) of DnaG reveal that within the hexamer the two domains of DnaB pack with strikingly different symmetries to form a distinct two-layered ring structure. Each of three bound HBDs stabilizes the DnaB hexamer in a conformation that may increase its processivity. Three positive, conserved electrostatic patches on the N-terminal domain of DnaB may also serve as a binding site for DNA and thereby guide the DNA to a DnaG active site.

About this StructureAbout this Structure

2R6E is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Structure of hexameric DnaB helicase and its complex with a domain of DnaG primase., Bailey S, Eliason WK, Steitz TA, Science. 2007 Oct 19;318(5849):459-63. PMID:17947583

Page seeded by OCA on Thu Mar 20 18:33:00 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2r6e.jpg|left|200px]]<br /><applet load="2r6e" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2r6e.jpg|left|200px]]
-caption="2r6e, resolution 5.019&Aring;" />
-'''Crystal Form B2'''<br />
+ {{Structure
+|PDB= 2r6e |SIZE=350|CAPTION= <scene name='initialview01'>2r6e</scene>, resolution 5.019&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
+|ACTIVITY=
+|GENE= DnaB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 Geobacillus stearothermophilus])
+}}
+'''Crystal Form B2'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-R6E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R6E OCA].
+R6E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R6E OCA].
 ==Reference==
-Structure of hexameric DnaB helicase and its complex with a domain of DnaG primase., Bailey S, Eliason WK, Steitz TA, Science. 2007 Oct 19;318(5849):459-63. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17947583 17947583]
+Structure of hexameric DnaB helicase and its complex with a domain of DnaG primase., Bailey S, Eliason WK, Steitz TA, Science. 2007 Oct 19;318(5849):459-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17947583 17947583]
 [[Category: Geobacillus stearothermophilus]]
 [[Category: Single protein]]
@@ Line 21: / Line 30: @@
 [[Category: replication dnab]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:44:55 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:33:00 2008''