2r4x: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2r4x.jpg|left|200px]] | [[Image:2r4x.jpg|left|200px]] | ||
'''Ligand Migration and Binding in The Dimeric Hemoglobin of Scapharca Inaequivalvis: H69V/I114M co complex''' | {{Structure | ||
|PDB= 2r4x |SIZE=350|CAPTION= <scene name='initialview01'>2r4x</scene>, resolution 2.10Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> and <scene name='pdbligand=CMO:CARBON MONOXIDE'>CMO</scene> | |||
|ACTIVITY= | |||
|GENE= HbI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6561 Scapharca inaequivalvis]) | |||
}} | |||
'''Ligand Migration and Binding in The Dimeric Hemoglobin of Scapharca Inaequivalvis: H69V/I114M co complex''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
2R4X is a [ | 2R4X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Scapharca_inaequivalvis Scapharca inaequivalvis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R4X OCA]. | ||
==Reference== | ==Reference== | ||
Ligand migration and binding in the dimeric hemoglobin of Scapharca inaequivalvis., Nienhaus K, Knapp JE, Palladino P, Royer WE Jr, Nienhaus GU, Biochemistry. 2007 Dec 11;46(49):14018-31. Epub 2007 Nov 15. PMID:[http:// | Ligand migration and binding in the dimeric hemoglobin of Scapharca inaequivalvis., Nienhaus K, Knapp JE, Palladino P, Royer WE Jr, Nienhaus GU, Biochemistry. 2007 Dec 11;46(49):14018-31. Epub 2007 Nov 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18001141 18001141] | ||
[[Category: Scapharca inaequivalvis]] | [[Category: Scapharca inaequivalvis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 31: | Line 40: | ||
[[Category: oxygen transport]] | [[Category: oxygen transport]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:32:33 2008'' | ||
Revision as of 19:32, 20 March 2008
| |||||||
| , resolution 2.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | HbI (Scapharca inaequivalvis) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Ligand Migration and Binding in The Dimeric Hemoglobin of Scapharca Inaequivalvis: H69V/I114M co complex
OverviewOverview
Using Fourier transform infrared (FTIR) spectroscopy combined with temperature derivative spectroscopy (TDS) at cryogenic temperatures, we have studied CO binding to the heme and CO migration among cavities in the interior of the dimeric hemoglobin of Scapharca inaequivalvis (HbI) after photodissociation. By combining these studies with X-ray crystallography, three transient ligand docking sites were identified: a primary docking site B in close vicinity to the heme iron, and two secondary docking sites C and D corresponding to the Xe4 and Xe2 cavities of myoglobin. To assess the relevance of these findings for physiological binding, we also performed flash photolysis experiments on HbICO at room temperature and equilibrium binding studies with dioxygen. Our results show that the Xe4 and Xe2 cavities serve as transient docking sites for unbound ligands in the protein, but not as way stations on the entry/exit pathway. For HbI, the so-called histidine gate mechanism proposed for other globins appears as a plausible entry/exit route as well.
About this StructureAbout this Structure
2R4X is a Single protein structure of sequence from Scapharca inaequivalvis. Full crystallographic information is available from OCA.
ReferenceReference
Ligand migration and binding in the dimeric hemoglobin of Scapharca inaequivalvis., Nienhaus K, Knapp JE, Palladino P, Royer WE Jr, Nienhaus GU, Biochemistry. 2007 Dec 11;46(49):14018-31. Epub 2007 Nov 15. PMID:18001141
Page seeded by OCA on Thu Mar 20 18:32:33 2008