2r4s: Difference between revisions
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'''Crystal structure of the human beta2 adrenoceptor''' | {{Structure | ||
|PDB= 2r4s |SIZE=350|CAPTION= <scene name='initialview01'>2r4s</scene>, resolution 3.400Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= ADRB2, ADRB2R, B2AR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |||
}} | |||
'''Crystal structure of the human beta2 adrenoceptor''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2R4S is a [ | 2R4S is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R4S OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of the human beta2 adrenergic G-protein-coupled receptor., Rasmussen SG, Choi HJ, Rosenbaum DM, Kobilka TS, Thian FS, Edwards PC, Burghammer M, Ratnala VR, Sanishvili R, Fischetti RF, Schertler GF, Weis WI, Kobilka BK, Nature. 2007 Nov 15;450(7168):383-7. Epub 2007 Oct 21. PMID:[http:// | Crystal structure of the human beta2 adrenergic G-protein-coupled receptor., Rasmussen SG, Choi HJ, Rosenbaum DM, Kobilka TS, Thian FS, Edwards PC, Burghammer M, Ratnala VR, Sanishvili R, Fischetti RF, Schertler GF, Weis WI, Kobilka BK, Nature. 2007 Nov 15;450(7168):383-7. Epub 2007 Oct 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17952055 17952055] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
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[[Category: transmembrane helix]] | [[Category: transmembrane helix]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:32:35 2008'' | ||
Revision as of 19:32, 20 March 2008
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| , resolution 3.400Å | |||||||
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| Gene: | ADRB2, ADRB2R, B2AR (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the human beta2 adrenoceptor
OverviewOverview
Structural analysis of G-protein-coupled receptors (GPCRs) for hormones and neurotransmitters has been hindered by their low natural abundance, inherent structural flexibility, and instability in detergent solutions. Here we report a structure of the human beta2 adrenoceptor (beta2AR), which was crystallized in a lipid environment when bound to an inverse agonist and in complex with a Fab that binds to the third intracellular loop. Diffraction data were obtained by high-brilliance microcrystallography and the structure determined at 3.4 A/3.7 A resolution. The cytoplasmic ends of the beta2AR transmembrane segments and the connecting loops are well resolved, whereas the extracellular regions of the beta2AR are not seen. The beta2AR structure differs from rhodopsin in having weaker interactions between the cytoplasmic ends of transmembrane (TM)3 and TM6, involving the conserved E/DRY sequences. These differences may be responsible for the relatively high basal activity and structural instability of the beta2AR, and contribute to the challenges in obtaining diffraction-quality crystals of non-rhodopsin GPCRs.
DiseaseDisease
Known diseases associated with this structure: Asthma, nocturnal, susceptibility to OMIM:[109690], Beta-2-adrenoreceptor agonist, reduced response to OMIM:[109690], Obesity, susceptibility to OMIM:[109690]
About this StructureAbout this Structure
2R4S is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the human beta2 adrenergic G-protein-coupled receptor., Rasmussen SG, Choi HJ, Rosenbaum DM, Kobilka TS, Thian FS, Edwards PC, Burghammer M, Ratnala VR, Sanishvili R, Fischetti RF, Schertler GF, Weis WI, Kobilka BK, Nature. 2007 Nov 15;450(7168):383-7. Epub 2007 Oct 21. PMID:17952055
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OCA- Pages with broken file links
- Homo sapiens
- Mus musculus
- Protein complex
- Burghammer, M.
- Choi, H J.
- Edwards, P C.
- Fischetti, R F.
- Kobilka, B K.
- Kobilka, T S.
- Rasmussen, S G.
- Ratnala, V R.
- Rosenbaum, D M.
- Sanishvili, R.
- Schertler, G F.
- Thian, F S.
- Weis, W I.
- G-protein coupled receptor
- Glycoprotein
- Lipoprotein
- Palmitate
- Phosphorylation
- Polymorphism
- Receptor
- Signaling protein
- Transducer
- Transmembrane helix