2r02: Difference between revisions

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Revision as of 19:31, 20 March 2008

File:2r02.jpg

, resolution 2.60Å

Gene:

PDCD6IP, AIP1, ALIX, KIAA1375 (Homo sapiens)

Coordinates:

save as pdb, mmCIF, xml

Crystal Structure of ALIX/AIP1 in complex with the HIV-1 YPLTSL Late Domain

OverviewOverview

Retrovirus budding requires short peptide motifs (late domains) located within the viral Gag protein that function by recruiting cellular factors. The YPX(n)L late domains of HIV and other lentiviruses recruit the protein ALIX (also known as AIP1), which also functions in vesicle formation at the multivesicular body and in the abscission stage of cytokinesis. Here, we report the crystal structures of ALIX in complex with the YPX(n)L late domains from HIV-1 and EIAV. The two distinct late domains bind at the same site on the ALIX V domain but adopt different conformations that allow them to make equivalent contacts. Binding studies and functional assays verified the importance of key interface residues and revealed that binding affinities are tuned by context-dependent effects. These results reveal how YPX(n)L late domains recruit ALIX to facilitate virus budding and how ALIX can bind YPX(n)L sequences with both n = 1 and n = 3.

About this StructureAbout this Structure

2R02 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural and functional studies of ALIX interactions with YPX(n)L late domains of HIV-1 and EIAV., Zhai Q, Fisher RD, Chung HY, Myszka DG, Sundquist WI, Hill CP, Nat Struct Mol Biol. 2008 Jan;15(1):43-9. Epub 2007 Dec 9. PMID:18066081

Page seeded by OCA on Thu Mar 20 18:31:18 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2r02.jpg|left|200px]]<br /><applet load="2r02" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2r02.jpg|left|200px]]
-caption="2r02, resolution 2.60&Aring;" />
-'''Crystal Structure of ALIX/AIP1 in complex with the HIV-1 YPLTSL Late Domain'''<br />
+ {{Structure
+|PDB= 2r02 |SIZE=350|CAPTION= <scene name='initialview01'>2r02</scene>, resolution 2.60&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE= PDCD6IP, AIP1, ALIX, KIAA1375 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+}}
+'''Crystal Structure of ALIX/AIP1 in complex with the HIV-1 YPLTSL Late Domain'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-R02 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R02 OCA].
+R02 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R02 OCA].
 ==Reference==
-Structural and functional studies of ALIX interactions with YPX(n)L late domains of HIV-1 and EIAV., Zhai Q, Fisher RD, Chung HY, Myszka DG, Sundquist WI, Hill CP, Nat Struct Mol Biol. 2008 Jan;15(1):43-9. Epub 2007 Dec 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18066081 18066081]
+Structural and functional studies of ALIX interactions with YPX(n)L late domains of HIV-1 and EIAV., Zhai Q, Fisher RD, Chung HY, Myszka DG, Sundquist WI, Hill CP, Nat Struct Mol Biol. 2008 Jan;15(1):43-9. Epub 2007 Dec 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18066081 18066081]
 [[Category: Homo sapiens]]
 [[Category: Protein complex]]
@@ Line 16: / Line 25: @@
 [[Category: Hill, C P.]]
 [[Category: Zhai, Q.]]
-[[Category: aids]]
+[[Category: aid]]
 [[Category: apoptosis]]
 [[Category: capsid protein]]
@@ Line 38: / Line 47: @@
 [[Category: zinc-finger]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:43:37 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:31:18 2008''