2qvj: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2qvj.jpg|left|200px]] | [[Image:2qvj.jpg|left|200px]] | ||
'''Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant''' | {{Structure | ||
|PDB= 2qvj |SIZE=350|CAPTION= <scene name='initialview01'>2qvj</scene>, resolution 2.800Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11277 Vesicular stomatitis Indiana virus]) | |||
}} | |||
'''Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
2QVJ is a [ | 2QVJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA]. | ||
==Reference== | ==Reference== | ||
Role of intermolecular interactions of vesicular stomatitis virus nucleoprotein in RNA encapsidation., Zhang X, Green TJ, Tsao J, Qiu S, Luo M, J Virol. 2008 Jan;82(2):674-82. Epub 2007 Nov 14. PMID:[http:// | Role of intermolecular interactions of vesicular stomatitis virus nucleoprotein in RNA encapsidation., Zhang X, Green TJ, Tsao J, Qiu S, Luo M, J Virol. 2008 Jan;82(2):674-82. Epub 2007 Nov 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18003727 18003727] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Vesicular stomatitis indiana virus]] | [[Category: Vesicular stomatitis indiana virus]] | ||
| Line 26: | Line 35: | ||
[[Category: virion]] | [[Category: virion]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:29:59 2008'' | ||
Revision as of 19:30, 20 March 2008
| |||||||
| , resolution 2.800Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | N (Vesicular stomatitis Indiana virus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant
OverviewOverview
The crystal structure of the vesicular stomatitis virus nucleoprotein (N) in complex with RNA reveals extensive and specific intermolecular interactions among the N molecules in the 10-member oligomer. What roles these interactions play in encapsidating RNA was studied by mutagenesis of the N protein. Three N mutants intended for disruption of the intermolecular interactions were designed and coexpressed with the phosphoprotein (P) in an Escherichia coli system previously described (T. J. Green et al., J. Virol. 74:9515-9524, 2000). Mutants N (Delta1-22), N (Delta347-352), and N (320-324, (Ala)(5)) lost RNA encapsidation and oligomerization but still bound with P. Another mutant, N (Ser290-->Trp), was able to form a stable ring-like N oligomer and bind with the P protein but was no longer able to encapsidate RNA. The crystal structure of N (Ser290-->Trp) at 2.8 A resolution showed that this mutant can maintain all the same intermolecular interactions as the wild-type N except for a slight unwinding of the N-terminal lobe. These results suggest that the intermolecular contacts among the N molecules are required for encapsidation of the viral RNA.
About this StructureAbout this Structure
2QVJ is a Single protein structure of sequence from Vesicular stomatitis indiana virus. Full crystallographic information is available from OCA.
ReferenceReference
Role of intermolecular interactions of vesicular stomatitis virus nucleoprotein in RNA encapsidation., Zhang X, Green TJ, Tsao J, Qiu S, Luo M, J Virol. 2008 Jan;82(2):674-82. Epub 2007 Nov 14. PMID:18003727
Page seeded by OCA on Thu Mar 20 18:29:59 2008