2qf7: Difference between revisions

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Revision as of 19:25, 20 March 2008

File:2qf7.jpg

, resolution 2.000Å

Ligands:

, , , , , and

Gene:

pyc (Rhizobium etli)

Activity:

Pyruvate carboxylase, with EC number 6.4.1.1

Coordinates:

save as pdb, mmCIF, xml

Crystal structure of a complete multifunctional pyruvate carboxylase from Rhizobium etli

OverviewOverview

Biotin-dependent multifunctional enzymes carry out metabolically important carboxyl group transfer reactions and are potential targets for the treatment of obesity and type 2 diabetes. These enzymes use a tethered biotin cofactor to carry an activated carboxyl group between distantly spaced active sites. The mechanism of this transfer has remained poorly understood. Here we report the complete structure of pyruvate carboxylase at 2.0 angstroms resolution, which shows its domain arrangement. The structure, when combined with mutagenic analysis, shows that intermediate transfer occurs between active sites on separate polypeptide chains. In addition, domain rearrangements associated with activator binding decrease the distance between active-site pairs, providing a mechanism for allosteric activation. This description provides insight into the function of biotin-dependent enzymes and presents a new paradigm for multifunctional enzyme catalysis.

About this StructureAbout this Structure

2QF7 is a Single protein structure of sequence from Rhizobium etli. Full crystallographic information is available from OCA.

ReferenceReference

Domain architecture of pyruvate carboxylase, a biotin-dependent multifunctional enzyme., St Maurice M, Reinhardt L, Surinya KH, Attwood PV, Wallace JC, Cleland WW, Rayment I, Science. 2007 Aug 24;317(5841):1076-9. PMID:17717183

Page seeded by OCA on Thu Mar 20 18:25:14 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2qf7.jpg|left|200px]]<br /><applet load="2qf7" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2qf7.jpg|left|200px]]
-caption="2qf7, resolution 2.000&Aring;" />
-'''Crystal structure of a complete multifunctional pyruvate carboxylase from Rhizobium etli'''<br />
+ {{Structure
+|PDB= 2qf7 |SIZE=350|CAPTION= <scene name='initialview01'>2qf7</scene>, resolution 2.000&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=SAP:ADENOSINE-5'-DIPHOSPHATE+MONOTHIOPHOSPHATE'>SAP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> and <scene name='pdbligand=FMT:FORMIC ACID'>FMT</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Pyruvate_carboxylase Pyruvate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.1 6.4.1.1]
+|GENE= pyc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29449 Rhizobium etli])
+}}
+'''Crystal structure of a complete multifunctional pyruvate carboxylase from Rhizobium etli'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-QF7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhizobium_etli Rhizobium etli] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=COA:'>COA</scene>, <scene name='pdbligand=SAP:'>SAP</scene>, <scene name='pdbligand=GOL:'>GOL</scene> and <scene name='pdbligand=FMT:'>FMT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pyruvate_carboxylase Pyruvate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.1 6.4.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QF7 OCA].
+QF7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhizobium_etli Rhizobium etli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QF7 OCA].
 ==Reference==
-Domain architecture of pyruvate carboxylase, a biotin-dependent multifunctional enzyme., St Maurice M, Reinhardt L, Surinya KH, Attwood PV, Wallace JC, Cleland WW, Rayment I, Science. 2007 Aug 24;317(5841):1076-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17717183 17717183]
+Domain architecture of pyruvate carboxylase, a biotin-dependent multifunctional enzyme., St Maurice M, Reinhardt L, Surinya KH, Attwood PV, Wallace JC, Cleland WW, Rayment I, Science. 2007 Aug 24;317(5841):1076-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17717183 17717183]
 [[Category: Pyruvate carboxylase]]
 [[Category: Rhizobium etli]]
@@ Line 29: / Line 38: @@
 [[Category: multi-functional]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:38:49 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:25:14 2008''