4r6r: Difference between revisions
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''' | ==Jacalin-carbohydrate interactions. Distortion of the ligand as a determinant of affinity.== | ||
<StructureSection load='4r6r' size='340' side='right' caption='[[4r6r]], [[Resolution|resolution]] 1.38Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4r6r]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R6R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R6R FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=147:1-O-[P-NITROPHENYL]-BETA-D-GALACTOPYRANOSE'>147</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1jac|1jac]], [[1ugw|1ugw]], [[1ku8|1ku8]], [[1m26|1m26]], [[1uh0|1uh0]], [[1ugy|1ugy]], [[4r6n|4r6n]], [[4r6o|4r6o]], [[4r6p|4r6p]], [[4r6q|4r6q]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r6r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r6r RCSB], [http://www.ebi.ac.uk/pdbsum/4r6r PDBsum]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/LECA_ARTIN LECA_ARTIN]] D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum. [[http://www.uniprot.org/uniprot/LECB3_ARTIN LECB3_ARTIN]] D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Jacalin, a tetrameric two-chain lectin (66,000 Mr) from jackfruit seeds, is highly specific for the tumour associated T-antigenic disaccharide. The crystal structure of jacalin with methyl-alpha-D-galactose reveals that each subunit has a three-fold symmetric beta-prism fold made up of three four-stranded beta-sheets. The lectin exhibits a novel carbohydrate-binding site involving the N terminus of the alpha-chain which is generated through a post-translational modification involving proteolysis, the first known instance where such a modification has been used to confer carbohydrate specificity. This new lectin fold may be characteristic of the Moraceae plant family. The structure provides an explanation for the relative affinities of the lectin for galactose derivatives and provides insights into the structural basis of its T-antigen specificity. | |||
A novel mode of carbohydrate recognition in jacalin, a Moraceae plant lectin with a beta-prism fold.,Sankaranarayanan R, Sekar K, Banerjee R, Sharma V, Surolia A, Vijayan M Nat Struct Biol. 1996 Jul;3(7):596-603. PMID:8673603<ref>PMID:8673603</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: Artocarpus integer]] | |||
[[Category: Abhinav, K V]] | |||
[[Category: Sharma, K]] | [[Category: Sharma, K]] | ||
[[Category: Surolia, A]] | [[Category: Surolia, A]] | ||
[[Category: | [[Category: Swaminathan, C P]] | ||
[[Category: Vijayan, M]] | |||
[[Category: Beta-prism i fold]] | |||
[[Category: Galactose and sugar binding protein]] | |||
[[Category: Galactose specific lectin]] | |||
[[Category: Plant lectin]] | |||
[[Category: Post translational proteolysis]] | |||
[[Category: Sugar binding protein]] | |||
[[Category: T-antigen binding protein]] | |||
Revision as of 15:54, 18 February 2015
Jacalin-carbohydrate interactions. Distortion of the ligand as a determinant of affinity.Jacalin-carbohydrate interactions. Distortion of the ligand as a determinant of affinity.
Structural highlights
Function[LECA_ARTIN] D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum. [LECB3_ARTIN] D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum. Publication Abstract from PubMedJacalin, a tetrameric two-chain lectin (66,000 Mr) from jackfruit seeds, is highly specific for the tumour associated T-antigenic disaccharide. The crystal structure of jacalin with methyl-alpha-D-galactose reveals that each subunit has a three-fold symmetric beta-prism fold made up of three four-stranded beta-sheets. The lectin exhibits a novel carbohydrate-binding site involving the N terminus of the alpha-chain which is generated through a post-translational modification involving proteolysis, the first known instance where such a modification has been used to confer carbohydrate specificity. This new lectin fold may be characteristic of the Moraceae plant family. The structure provides an explanation for the relative affinities of the lectin for galactose derivatives and provides insights into the structural basis of its T-antigen specificity. A novel mode of carbohydrate recognition in jacalin, a Moraceae plant lectin with a beta-prism fold.,Sankaranarayanan R, Sekar K, Banerjee R, Sharma V, Surolia A, Vijayan M Nat Struct Biol. 1996 Jul;3(7):596-603. PMID:8673603[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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