2pyp: Difference between revisions

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File:2pyp.jpg

, resolution 1.9Å

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PHOTOACTIVE YELLOW PROTEIN, PHOTOSTATIONARY STATE, 50% GROUND STATE, 50% BLEACHED

OverviewOverview

The blue-light photoreceptor photoactive yellow protein (PYP) undergoes a self-contained light cycle. The atomic structure of the bleached signaling intermediate in the light cycle of PYP was determined by millisecond time-resolved, multiwavelength Laue crystallography and simultaneous optical spectroscopy. Light-induced trans-to-cis isomerization of the 4-hydroxycinnamyl chromophore and coupled protein rearrangements produce a new set of active-site hydrogen bonds. An arginine gateway opens, allowing solvent exposure and protonation of the chromophore's phenolic oxygen. Resulting changes in shape, hydrogen bonding, and electrostatic potential at the protein surface form a likely basis for signal transduction. The structural results suggest a general framework for the interpretation of protein photocycles.

About this StructureAbout this Structure

2PYP is a Single protein structure of sequence from Halorhodospira halophila. Full crystallographic information is available from OCA.

ReferenceReference

Structure of a protein photocycle intermediate by millisecond time-resolved crystallography., Genick UK, Borgstahl GE, Ng K, Ren Z, Pradervand C, Burke PM, Srajer V, Teng TY, Schildkamp W, McRee DE, Moffat K, Getzoff ED, Science. 1997 Mar 7;275(5305):1471-5. PMID:9045611

Page seeded by OCA on Thu Mar 20 18:19:37 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2pyp.jpg|left|200px]]<br /><applet load="2pyp" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2pyp.jpg|left|200px]]
-caption="2pyp, resolution 1.9&Aring;" />
-'''PHOTOACTIVE YELLOW PROTEIN, PHOTOSTATIONARY STATE, 50% GROUND STATE, 50% BLEACHED'''<br />
+ {{Structure
+|PDB= 2pyp |SIZE=350|CAPTION= <scene name='initialview01'>2pyp</scene>, resolution 1.9&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=HC4:4'-HYDROXYCINNAMIC ACID'>HC4</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''PHOTOACTIVE YELLOW PROTEIN, PHOTOSTATIONARY STATE, 50% GROUND STATE, 50% BLEACHED'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-PYP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila] with <scene name='pdbligand=HC4:'>HC4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PYP OCA].
+PYP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PYP OCA].
 ==Reference==
-Structure of a protein photocycle intermediate by millisecond time-resolved crystallography., Genick UK, Borgstahl GE, Ng K, Ren Z, Pradervand C, Burke PM, Srajer V, Teng TY, Schildkamp W, McRee DE, Moffat K, Getzoff ED, Science. 1997 Mar 7;275(5305):1471-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9045611 9045611]
+Structure of a protein photocycle intermediate by millisecond time-resolved crystallography., Genick UK, Borgstahl GE, Ng K, Ren Z, Pradervand C, Burke PM, Srajer V, Teng TY, Schildkamp W, McRee DE, Moffat K, Getzoff ED, Science. 1997 Mar 7;275(5305):1471-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9045611 9045611]
 [[Category: Halorhodospira halophila]]
 [[Category: Single protein]]
@@ Line 30: / Line 39: @@
 [[Category: photoreceptor]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:34:17 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:19:37 2008''