4fof: Difference between revisions

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-{{STRUCTURE_4fof|  PDB=4fof  |  SCENE=  }}
+==Crystal Structure of the blue-light absorbing form of the Thermosynechococcus elongatus PixJ GAF-domain==
-===Crystal Structure of the blue-light absorbing form of the Thermosynechococcus elongatus PixJ GAF-domain===
+<StructureSection load='4fof' size='340' side='right' caption='[[4fof]], [[Resolution|resolution]] 2.42&Aring;' scene=''>
-{{ABSTRACT_PUBMED_23219880}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4fof]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Theeb Theeb] and [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus_bp-1 Thermosynechococcus elongatus bp-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FOF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FOF FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=VRB:PHYCOVIOLOBILIN,+BLUE+LIGHT-ABSORBING+FORM'>VRB</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tll0569 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 Thermosynechococcus elongatus BP-1]), HO1 and PcyA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 THEEB])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fof OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fof RCSB], [http://www.ebi.ac.uk/pdbsum/4fof PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The phytochrome superfamily encompasses a diverse collection of photochromic photoreceptors in plants and microorganisms that employ a covalently linked bilin cradled in a cGMP-phosphodiesterase/adenylyl-cyclase/FhlA (GAF) domain to detect light. Whereas most interconvert between red- and far-red-light-absorbing states, cyanobacteria also express variants called cyanobacteriochromes (CBCRs) that modify bilin absorption to collectively perceive the entire visible spectrum. Here, we present two X-ray crystallographic structures of the GAF domain from the blue/green photochromic CBCR PixJ from Thermosynechococcus elongatus. These structures confirm the hypothesis that CBCRs variably manipulate the chromophore pi-conjugation system through isomerization and a second thioether linkage, in this case involving the bilin C10 carbon and Cys494 within a DXCF sequence characteristic of blue/green CBCRs. Biochemical studies support a mechanism for photoconversion whereby the second linkage ruptures on route to the green-light-absorbing state. Collectively, the TePixJ(GAF) models illustrate the remarkable structural and photochemical versatility among phytochromes and CBCRs in driving light perception.
-==About this Structure==
+A Photo-Labile Thioether Linkage to Phycoviolobilin Provides the Foundation for the Blue/Green Photocycles in DXCF-Cyanobacteriochromes.,Burgie ES, Walker JM, Phillips GN Jr, Vierstra RD Structure. 2013 Jan 8;21(1):88-97. doi: 10.1016/j.str.2012.11.001. Epub 2012 Dec , 6. PMID:23219880<ref>PMID:23219880</ref>
-[[4fof]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus_bp-1 Thermosynechococcus elongatus bp-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FOF OCA].
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+==See Also==
+*[[Chemotaxis protein|Chemotaxis protein]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Theeb]]
 [[Category: Thermosynechococcus elongatus bp-1]]
-[[Category: Burgie, E S.]]
+[[Category: Burgie, E S]]
-[[Category: Phillips, G N.]]
+[[Category: Phillips, G N]]
-[[Category: Vierstra, R D.]]
+[[Category: Vierstra, R D]]
-[[Category: Walker, J M.]]
+[[Category: Walker, J M]]
 [[Category: Blue/green photoreceptor]]
 [[Category: Cyanobacteriochrome]]