4x4t: Difference between revisions

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'''Unreleased structure'''
==Crystal structure of the A.fulgidus CCA-adding enzyme in complex with a G70A arginyl-tRNA minihelix ending in CCACCA==
 
<StructureSection load='4x4t' size='340' side='right' caption='[[4x4t]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
The entry 4x4t is ON HOLD  until Paper Publication
== Structural highlights ==
 
<table><tr><td colspan='2'>[[4x4t]] is a 9 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X4T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X4T FirstGlance]. <br>
Authors: Kuhn, C.-D., Joshua-Tor, L.
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
 
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5BU:5-BROMO-URIDINE-5-MONOPHOSPHATE'>5BU</scene></td></tr>
Description: Crystal structure of the A.fulgidus CCA-adding enzyme in complex with a G70A arginyl-tRNA minihelix ending in CCACCA
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/CCA_tRNA_nucleotidyltransferase CCA tRNA nucleotidyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.72 2.7.7.72] </span></td></tr>
[[Category: Unreleased Structures]]
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x4t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x4t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4x4t RCSB], [http://www.ebi.ac.uk/pdbsum/4x4t PDBsum]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/CCA_ARCFU CCA_ARCFU]] Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.<ref>PMID:14592988</ref> 
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: CCA tRNA nucleotidyltransferase]]
[[Category: Joshua-Tor, L]]
[[Category: Joshua-Tor, L]]
[[Category: Kuhn, C.-D]]
[[Category: Kuhn, C D]]
[[Category: Cca-adding enzyme]]
[[Category: Ncrna]]
[[Category: Non-coding rna]]
[[Category: Nucleotidyltransferase]]
[[Category: Protein-rna complex]]
[[Category: Transferase-rna complex]]
[[Category: Trna]]

Revision as of 15:05, 12 February 2015

Crystal structure of the A.fulgidus CCA-adding enzyme in complex with a G70A arginyl-tRNA minihelix ending in CCACCACrystal structure of the A.fulgidus CCA-adding enzyme in complex with a G70A arginyl-tRNA minihelix ending in CCACCA

Structural highlights

4x4t is a 9 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:,
NonStd Res:
Activity:CCA tRNA nucleotidyltransferase, with EC number 2.7.7.72
Resources:FirstGlance, OCA, RCSB, PDBsum

Function

[CCA_ARCFU] Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.[1]

References

  1. ↑ Okabe M, Tomita K, Ishitani R, Ishii R, Takeuchi N, Arisaka F, Nureki O, Yokoyama S. Divergent evolutions of trinucleotide polymerization revealed by an archaeal CCA-adding enzyme structure. EMBO J. 2003 Nov 3;22(21):5918-27. PMID:14592988 doi:http://dx.doi.org/10.1093/emboj/cdg563

4x4t, resolution 2.50Å

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