4rvh: Difference between revisions

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-'''Unreleased structure'''
+==Crystal structure of MtmC in complex with SAH and TDP-4-keto-D-olivose==
+<StructureSection load='4rvh' size='340' side='right' caption='[[4rvh]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4rvh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RVH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RVH FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3YN:[(2R,3S,5R)-3-HYDROXY-5-(5-METHYL-2,4-DIOXO-3,4-DIHYDROPYRIMIDIN-1(2H)-YL)TETRAHYDROFURAN-2-YL]METHYL+(2R,4R,6R)-4-HYDROXY-6-METHYL-5-OXOTETRAHYDRO-2H-PYRAN-2-YL+DIHYDROGEN+DIPHOSPHATE'>3YN</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1rv9|1rv9]], [[4rvd|4rvd]], [[4rvf|4rvf]], [[4rvg|4rvg]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rvh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rvh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rvh RCSB], [http://www.ebi.ac.uk/pdbsum/4rvh PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+More and more post-PKS tailoring enzymes are recognized to be multifunctional and co-dependent on other tailoring enzymes. One of the recently discovered intriguing examples is MtmC, a bifunctional TDP-4-keto-D-olivose ketoreductase-methyltransferase, which - in co-dependence with glycosyltransferase MtmGIV - is a key contributor to the biosynthesis of the critical trisaccharide chain of the antitumor antibiotic mithramycin (MTM), produced by Streptomyces argillaceus. We report crystal structures of three binary complexes of MtmC with its methylation co-substrate SAM, its co-product SAH, and a nucleotide TDP as well as crystal structures of two ternary complexes, MtmC-SAH-TDP-4-keto-D-olivose and MtmC-SAM-TDP, in the range of 2.2-2.7 A in resolution. The structures reveal general and sugar-specific recognition and catalytic structural features of MtmC. Depending on the catalytic function that is carried out by MtmC, it must bind either NADPH or SAM in the same co-factor binding pocket. A tyrosine residue (Tyr79) appears as a lid covering the sugar moiety of the substrate during the methyl transfer reaction. This residue swings out of the active site by about 180o in the absence of the substrate. This unique conformational change likely serves to release the methylated product and, possibly, to open up the active site for binding the bulkier co-substrate NADPH prior to the reduction reaction.
-The entry 4rvh is ON HOLD  until Paper Publication
+Structural insight into MtmC, a bifunctional ketoreductase-methyltransferase involved in the assembly of the mithramycin trisaccharide chain.,Chen JM, Hou C, Wang G, Tsodikov OV, Rohr J Biochemistry. 2015 Jan 14. PMID:25587924<ref>PMID:25587924</ref>
-Authors: Tsodikov, O.V., Hou, C., Chen, J.-M., Rohr, J.
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-Description: Crystal structure of MC in complex with SAH and substrate
+== References ==
-[[Category: Unreleased Structures]]
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Chen, J M]]
+[[Category: Hou, C]]
 [[Category: Rohr, J]]
-[[Category: Tsodikov, O.V]]
+[[Category: Tsodikov, O V]]
-[[Category: Chen, J.-M]]
+[[Category: C-methyltransferase]]
-[[Category: Hou, C]]
+[[Category: Sam]]
+[[Category: Tdp-4-keto-d-olivose]]
+[[Category: Transferase]]