2p6r: Difference between revisions
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[[Image:2p6r.gif|left|200px]] | [[Image:2p6r.gif|left|200px]] | ||
'''Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA''' | {{Structure | ||
|PDB= 2p6r |SIZE=350|CAPTION= <scene name='initialview01'>2p6r</scene>, resolution 3.000Å | |||
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'''Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2P6R is a [ | 2P6R is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6R OCA]. | ||
==Reference== | ==Reference== | ||
Structural basis for DNA duplex separation by a superfamily-2 helicase., Buttner K, Nehring S, Hopfner KP, Nat Struct Mol Biol. 2007 Jul;14(7):647-52. Epub 2007 Jun 10. PMID:[http:// | Structural basis for DNA duplex separation by a superfamily-2 helicase., Buttner K, Nehring S, Hopfner KP, Nat Struct Mol Biol. 2007 Jul;14(7):647-52. Epub 2007 Jun 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17558417 17558417] | ||
[[Category: Archaeoglobus fulgidus]] | [[Category: Archaeoglobus fulgidus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: sf2 helicase]] | [[Category: sf2 helicase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:09:23 2008'' | ||
Revision as of 19:09, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA
OverviewOverview
To reveal the mechanism of processive strand separation by superfamily-2 (SF2) 3'-->5' helicases, we determined apo and DNA-bound crystal structures of archaeal Hel308, a helicase that unwinds lagging strands and is related to human DNA polymerase theta. Our structure captures the duplex-unwinding reaction, shows that initial strand separation does not require ATP and identifies a prominent beta-hairpin loop as the unwinding element. Similar loops in hepatitis C virus NS3 helicase and RNA-decay factors support the idea that this duplex-unwinding mechanism is applicable to a broad subset of SF2 helicases. Comparison with ATP-bound SF2 enzymes suggests that ATP promotes processive unwinding of 1 base pair by ratchet-like transport of the 3' product strand. Our results provide a first structural framework for strand separation by processive SF2 3'-->5' helicases and reveal important mechanistic differences from SF1 helicases.
About this StructureAbout this Structure
2P6R is a Protein complex structure of sequences from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for DNA duplex separation by a superfamily-2 helicase., Buttner K, Nehring S, Hopfner KP, Nat Struct Mol Biol. 2007 Jul;14(7):647-52. Epub 2007 Jun 10. PMID:17558417
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