1e6e: Difference between revisions

The steroid hydroxylating system of adrenal cortex mitochondria consists, of the membrane-attached NADPH-dependent adrenodoxin reductase (AR), the, soluble one-electron transport protein adrenodoxin (Adx), and a, membrane-integrated cytochrome P450 of the CYP11 family. In the 2.3-A, resolution crystal structure of the Adx.AR complex, 580 A(2) of partly, polar surface are buried. Main interaction sites are centered around, Asp(79), Asp(76), Asp(72), and Asp(39) of Adx and around Arg(211), Arg(240), Arg(244), and Lys(27) of AR, respectively. In particular, the, region around Asp(39) defines a new protein interaction site for Adx, similar to those found in plant and bacterial ferredoxins. Additional, contacts involve the electron transfer region between the redox centers of, AR and Adx and C-terminal residues of Adx. The Adx residues Asp(113) to, Arg(115) adopt 3(10)-helical conformation and engage in loose, intermolecular contacts within a deep cleft of AR. Complex formation is, accompanied by a slight domain rearrangement in AR. The [2Fe-2S] cluster, of Adx and the isoalloxazine rings of FAD of AR are 10 A apart suggesting, a possible electron transfer route between these redox centers. The AR.Adx, complex represents the first structure of a biologically relevant complex, between a ferredoxin and its reductase.

1E6E is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with SO4, FAD and FES as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1E6E with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb82_1.html Cytochrome p450]]. Active as [http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] Structure known Active Sites: AC1, AC5, AC6 and AC9. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E6E OCA].  

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 14:08:15 2007''