Mycobacterium tuberculosis ArfA Rv0899: Difference between revisions
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2. Bacterium's adaptation to the acidic environment of the phagosome during infection by: | 2. Bacterium's adaptation to the acidic environment of the phagosome during infection by: | ||
a)deamidation of the amino acid pair <scene name='61/612805/Asn111_and_gly112/1'> Asn111-Gly112 </scene>, located at the end of α1 and preceding L3, a pH-dependent reaction whereby Asn is converted to Asp and ammonia is released. Asparagine residues preceding glycine, and situated in conformationally flexible regions of proteins, are frequently deamidated, with potentially significant consequences for protein regulation and function <ref>PMID: 20199110</ref> [[Image:Asparaginase-reaction.jpg|250px]] | a) deamidation of the amino acid pair <scene name='61/612805/Asn111_and_gly112/1'> Asn111-Gly112 </scene>, located at the end of α1 and preceding L3, a pH-dependent reaction whereby Asn is converted to Asp and ammonia is released. Asparagine residues preceding glycine, and situated in conformationally flexible regions of proteins, are frequently deamidated, with potentially significant consequences for protein regulation and function <ref>PMID: 20199110</ref>. [[Image:Asparaginase-reaction.jpg|250px]] | ||
b) pH-dependent conformational dynamics of hydrophobic cluster of 232, 225, 240, 244, 281, 285 <scene name='61/612805/D236_before_mutation/1'>in neutral pH (D236) </scene> and a more ordered structure at <scene name='61/612805/D236a_after_mut/3'>acidic pH (D236A) </scene>. | |||
Its functions in acid stress protection and [peptidoglycan][http://en.wikipedia.org/wiki/Peptidoglycan] binding site <scene name='61/612805/Peptidoglycan_binding_site/1'>(R277, R319, T261, D262, N270)</scene> suggest a link between the acid stress response and the physicochemical properties of the mycobacterial cell wall. These residues are strictly conserved in the OmpA -like family <ref>PMID: 22206986 </ref>. | Its functions in acid stress protection and [peptidoglycan][http://en.wikipedia.org/wiki/Peptidoglycan] binding site <scene name='61/612805/Peptidoglycan_binding_site/1'>(R277, R319, T261, D262, N270)</scene> suggest a link between the acid stress response and the physicochemical properties of the mycobacterial cell wall. These residues are strictly conserved in the OmpA -like family <ref>PMID: 22206986 </ref>. | ||