Mycobacterium tuberculosis ArfA Rv0899: Difference between revisions
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Asparagine is the primary ammonia source for ''Mycobacterium tuberculosis'' H37Rv at acidic pH <ref>PMID: 21410778 </ref>. | Asparagine is the primary ammonia source for ''Mycobacterium tuberculosis'' H37Rv at acidic pH <ref>PMID: 21410778 </ref>. | ||
The deletion of this gene impairs the uptake of some water-soluble substances, such as serine, glucose, and glycerol <ref>PMID: 12366842 </ref>. | The deletion of this gene impairs the uptake of some water-soluble substances, such as serine, glucose, and glycerol <ref>PMID: 12366842 </ref>. | ||
== Structural highlights == | == Structural highlights == | ||
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==Putative functions== | ==Putative functions== | ||
1. Acquisition of Zn(2+) ions. | 1. Acquisition of Zn(2+) ions by {{Template:ColorKey Composition Ligand}} L82, D96, F97, H125, D127 and V129. | ||
Using [[NMR]] chemical shift perturbation and isothermal calorimetric titration assays, Rv0899 was able to interact with <scene name='61/612805/Binding-site_for_zn/1'>Zn(2+) ions</scene>, which may indicate a role for Rv0899 in the process of Zn(2+) acquisition. | Using [[NMR]] chemical shift perturbation and isothermal calorimetric titration assays, Rv0899 was able to interact with <scene name='61/612805/Binding-site_for_zn/1'>Zn(2+) ions</scene>, which may indicate a role for Rv0899 in the process of Zn(2+) acquisition. | ||
<ref>PMID: 22108166 </ref> | <ref>PMID: 22108166 </ref> | ||