2ont: Difference between revisions
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[[Image:2ont.gif|left|200px]] | [[Image:2ont.gif|left|200px]] | ||
'''A swapped dimer of the HIV-1 capsid C-terminal domain''' | {{Structure | ||
|PDB= 2ont |SIZE=350|CAPTION= <scene name='initialview01'>2ont</scene>, resolution 2.400Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= gag-pol ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 Human immunodeficiency virus 1]) | |||
}} | |||
'''A swapped dimer of the HIV-1 capsid C-terminal domain''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2ONT is a [ | 2ONT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ONT OCA]. | ||
==Reference== | ==Reference== | ||
Domain-swapped dimerization of the HIV-1 capsid C-terminal domain., Ivanov D, Tsodikov OV, Kasanov J, Ellenberger T, Wagner G, Collins T, Proc Natl Acad Sci U S A. 2007 Mar 13;104(11):4353-8. Epub 2007 Mar 5. PMID:[http:// | Domain-swapped dimerization of the HIV-1 capsid C-terminal domain., Ivanov D, Tsodikov OV, Kasanov J, Ellenberger T, Wagner G, Collins T, Proc Natl Acad Sci U S A. 2007 Mar 13;104(11):4353-8. Epub 2007 Mar 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17360528 17360528] | ||
[[Category: Human immunodeficiency virus 1]] | [[Category: Human immunodeficiency virus 1]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: hiv; capsid; gag; domain swap]] | [[Category: hiv; capsid; gag; domain swap]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:02:06 2008'' | ||
Revision as of 19:02, 20 March 2008
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| , resolution 2.400Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | gag-pol (Human immunodeficiency virus 1) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
A swapped dimer of the HIV-1 capsid C-terminal domain
OverviewOverview
Assembly of the HIV and other retroviruses is primarily driven by the oligomerization of the Gag polyprotein, the major viral structural protein capable of forming virus-like particles even in the absence of all other virally encoded components. Several critical determinants of Gag oligomerization are located in the C-terminal domain of the capsid protein (CA-CTD), which encompasses the most conserved segment in the highly variable Gag protein called the major homology region (MHR). The CA-CTD is thought to function as a dimerization module, although the existing model of CA-CTD dimerization does not readily explain why the conserved residues of the MHR are essential for retroviral assembly. Here we describe an x-ray structure of a distinct domain-swapped variant of the HIV-1 CA-CTD dimer stabilized by a single amino acid deletion. In the domain-swapped structure, the MHR-containing segment forms a major part of the dimerization interface, providing a structural mechanism for the enigmatic function of the MHR in HIV assembly. Our observations suggest that swapping of the MHR segments of adjacent Gag molecules may be a critical intermediate in retroviral assembly.
About this StructureAbout this Structure
2ONT is a Single protein structure of sequence from Human immunodeficiency virus 1. Full crystallographic information is available from OCA.
ReferenceReference
Domain-swapped dimerization of the HIV-1 capsid C-terminal domain., Ivanov D, Tsodikov OV, Kasanov J, Ellenberger T, Wagner G, Collins T, Proc Natl Acad Sci U S A. 2007 Mar 13;104(11):4353-8. Epub 2007 Mar 5. PMID:17360528
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