4plx: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4plx]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PLX FirstGlance]. <br> | <table><tr><td colspan='2'>[[4plx]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PLX FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A23:ADENOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>A23</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr> | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A23:ADENOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>A23</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr> | ||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4plx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4plx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4plx RCSB], [http://www.ebi.ac.uk/pdbsum/4plx PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4plx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4plx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4plx RCSB], [http://www.ebi.ac.uk/pdbsum/4plx PDBsum]</span></td></tr> | ||
<table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Brown, J A | [[Category: Brown, J A]] | ||
[[Category: Bulkley, D | [[Category: Bulkley, D]] | ||
[[Category: Steitz, J A | [[Category: Steitz, J A]] | ||
[[Category: Steitz, T A | [[Category: Steitz, T A]] | ||
[[Category: Valenstein, M L | [[Category: Valenstein, M L]] | ||
[[Category: Wang, J | [[Category: Wang, J]] | ||
[[Category: Yario, T A | [[Category: Yario, T A]] | ||
[[Category: Long noncoding rna]] | [[Category: Long noncoding rna]] | ||
[[Category: Malat1]] | [[Category: Malat1]] | ||
Revision as of 13:29, 20 January 2015
Crystal structure of the triple-helical stability element at the 3' end of MALAT1Crystal structure of the triple-helical stability element at the 3' end of MALAT1
Structural highlights
Publication Abstract from PubMedMetastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a highly abundant nuclear long noncoding RNA that promotes malignancy. A 3'-stem-loop structure is predicted to confer stability by engaging a downstream A-rich tract in a triple helix, similar to the expression and nuclear retention element (ENE) from the KSHV polyadenylated nuclear RNA. The 3.1-A-resolution crystal structure of the human MALAT1 ENE and A-rich tract reveals a bipartite triple helix containing stacks of five and four U*A-U triples separated by a C(+)*G-C triplet and C-G doublet, extended by two A-minor interactions. In vivo decay assays indicate that this blunt-ended triple helix, with the 3' nucleotide in a U*A-U triple, inhibits rapid nuclear RNA decay. Interruption of the triple helix by the C-G doublet induces a 'helical reset' that explains why triple-helical stacks longer than six do not occur in nature. Structural insights into the stabilization of MALAT1 noncoding RNA by a bipartite triple helix.,Brown JA, Bulkley D, Wang J, Valenstein ML, Yario TA, Steitz TA, Steitz JA Nat Struct Mol Biol. 2014 Jul;21(7):633-40. doi: 10.1038/nsmb.2844. Epub 2014 Jun, 22. PMID:24952594[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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