3udc: Difference between revisions
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==Crystal structure of a membrane protein== | |||
=== | <StructureSection load='3udc' size='340' side='right' caption='[[3udc]], [[Resolution|resolution]] 3.35Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3udc]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UDC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UDC FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3t9n|3t9n]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MscS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3udc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3udc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3udc RCSB], [http://www.ebi.ac.uk/pdbsum/3udc PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Mechanosensitive (MS) channels are universal cellular membrane pores. Bacterial MS channels, as typified by MS channel of small conductance (MscS) from Escherichia coli (EcMscS), release osmolytes under hypoosmotic conditions. MS channels are known to be ion selective to different extents, but the underlying mechanism remains poorly understood. Here we identify an anion-selective MscS channel from Thermoanaerobacter tengcongensis (TtMscS). The structure of TtMscS closely resembles that of EcMscS, but it lacks the large cytoplasmic equatorial portals found in EcMscS. In contrast, the cytoplasmic pore formed by the C-terminal beta-barrel of TtMscS is larger than that of EcMscS and has a strikingly different pattern of electrostatic surface potential. Swapping the beta-barrel region between TtMscS and EcMscS partially switches the ion selectivity. Our study defines the role of the beta-barrel in the ion selection of an anion-selective MscS channel and provides a structural basis for understanding the ion selectivity of MscS channels. | |||
Structure and molecular mechanism of an anion-selective mechanosensitive channel of small conductance.,Zhang X, Wang J, Feng Y, Ge J, Li W, Sun W, Iscla I, Yu J, Blount P, Li Y, Yang M Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):18180-5. doi:, 10.1073/pnas.1207977109. Epub 2012 Oct 16. PMID:23074248<ref>PMID:23074248</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
[[Category: Ge, J | == References == | ||
[[Category: Li, W | <references/> | ||
[[Category: Yang, M | __TOC__ | ||
</StructureSection> | |||
[[Category: Ecoli]] | |||
[[Category: Ge, J]] | |||
[[Category: Li, W]] | |||
[[Category: Yang, M]] | |||
[[Category: Channel]] | [[Category: Channel]] | ||
[[Category: Mechanosensitive]] | [[Category: Mechanosensitive]] | ||
[[Category: Membrane protein]] | [[Category: Membrane protein]] | ||
Revision as of 11:14, 20 January 2015
Crystal structure of a membrane proteinCrystal structure of a membrane protein
Structural highlights
Publication Abstract from PubMedMechanosensitive (MS) channels are universal cellular membrane pores. Bacterial MS channels, as typified by MS channel of small conductance (MscS) from Escherichia coli (EcMscS), release osmolytes under hypoosmotic conditions. MS channels are known to be ion selective to different extents, but the underlying mechanism remains poorly understood. Here we identify an anion-selective MscS channel from Thermoanaerobacter tengcongensis (TtMscS). The structure of TtMscS closely resembles that of EcMscS, but it lacks the large cytoplasmic equatorial portals found in EcMscS. In contrast, the cytoplasmic pore formed by the C-terminal beta-barrel of TtMscS is larger than that of EcMscS and has a strikingly different pattern of electrostatic surface potential. Swapping the beta-barrel region between TtMscS and EcMscS partially switches the ion selectivity. Our study defines the role of the beta-barrel in the ion selection of an anion-selective MscS channel and provides a structural basis for understanding the ion selectivity of MscS channels. Structure and molecular mechanism of an anion-selective mechanosensitive channel of small conductance.,Zhang X, Wang J, Feng Y, Ge J, Li W, Sun W, Iscla I, Yu J, Blount P, Li Y, Yang M Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):18180-5. doi:, 10.1073/pnas.1207977109. Epub 2012 Oct 16. PMID:23074248[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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