2o67: Difference between revisions
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[[Image:2o67.jpg|left|200px]] | [[Image:2o67.jpg|left|200px]] | ||
'''Crystal structure of Arabidopsis thaliana PII bound to malonate''' | {{Structure | ||
|PDB= 2o67 |SIZE=350|CAPTION= <scene name='initialview01'>2o67</scene>, resolution 2.50Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MLI:MALONATE ION'>MLI</scene> | |||
|ACTIVITY= | |||
|GENE= AT4g01900, T7B11.16 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]) | |||
}} | |||
'''Crystal structure of Arabidopsis thaliana PII bound to malonate''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2O67 is a [ | 2O67 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O67 OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of Arabidopsis PII reveals novel structural elements unique to plants., Mizuno Y, Berenger B, Moorhead GB, Ng KK, Biochemistry. 2007 Feb 13;46(6):1477-83. PMID:[http:// | Crystal structure of Arabidopsis PII reveals novel structural elements unique to plants., Mizuno Y, Berenger B, Moorhead GB, Ng KK, Biochemistry. 2007 Feb 13;46(6):1477-83. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17279613 17279613] | ||
[[Category: Arabidopsis thaliana]] | [[Category: Arabidopsis thaliana]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: regulation of nitrogen and carbon metabolism]] | [[Category: regulation of nitrogen and carbon metabolism]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:55:36 2008'' | ||
Revision as of 18:55, 20 March 2008
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| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | AT4g01900, T7B11.16 (Arabidopsis thaliana) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Arabidopsis thaliana PII bound to malonate
OverviewOverview
The 1.9 A resolution crystal structure of PII from Arabidopsis thaliana reveals for the first time the molecular structure of a widely conserved regulator of carbon and nitrogen metabolism from a eukaryote. The structure provides a framework for understanding the arrangement of highly conserved residues shared with PII proteins from bacteria, archaea, and red algae as well as residues conserved only in plant PII. Most strikingly, a highly conserved segment at the N-terminus that is found only in plant PII forms numerous interactions with the alpha2 helix and projects from the surface of the homotrimer opposite to that occupied by the T-loop. In addition, solvent-exposed residues near the T-loop are highly conserved in plants but differ in prokaryotes. Several residues at the C-terminus that are also highly conserved only in plants contribute part of the ATP-binding site and likely participate in an ATP-induced conformational change. Structures of PII also reveal how citrate and malonate bind near the triphosphate binding site occupied by ATP in bacterial and archaeal PII proteins.
About this StructureAbout this Structure
2O67 is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of Arabidopsis PII reveals novel structural elements unique to plants., Mizuno Y, Berenger B, Moorhead GB, Ng KK, Biochemistry. 2007 Feb 13;46(6):1477-83. PMID:17279613
Page seeded by OCA on Thu Mar 20 17:55:36 2008