2o0f: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2o0f.jpg|left|200px]] | [[Image:2o0f.jpg|left|200px]] | ||
'''Docking of the modified RF3 X-ray structure into cryo-EM map of E.coli 70S ribosome bound with RF3''' | {{Structure | ||
|PDB= 2o0f |SIZE=350|CAPTION= <scene name='initialview01'>2o0f</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Docking of the modified RF3 X-ray structure into cryo-EM map of E.coli 70S ribosome bound with RF3''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
2O0F is a [ | 2O0F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O0F OCA]. | ||
==Reference== | ==Reference== | ||
RF3 induces ribosomal conformational changes responsible for dissociation of class I release factors., Gao H, Zhou Z, Rawat U, Huang C, Bouakaz L, Wang C, Cheng Z, Liu Y, Zavialov A, Gursky R, Sanyal S, Ehrenberg M, Frank J, Song H, Cell. 2007 Jun 1;129(5):929-41. PMID:[http:// | RF3 induces ribosomal conformational changes responsible for dissociation of class I release factors., Gao H, Zhou Z, Rawat U, Huang C, Bouakaz L, Wang C, Cheng Z, Liu Y, Zavialov A, Gursky R, Sanyal S, Ehrenberg M, Frank J, Song H, Cell. 2007 Jun 1;129(5):929-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17540173 17540173] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 30: | Line 39: | ||
[[Category: ribosome]] | [[Category: ribosome]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:53:28 2008'' | ||
Revision as of 18:53, 20 March 2008
| |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Docking of the modified RF3 X-ray structure into cryo-EM map of E.coli 70S ribosome bound with RF3
OverviewOverview
During translation termination, class II release factor RF3 binds to the ribosome to promote rapid dissociation of a class I release factor (RF) in a GTP-dependent manner. We present the crystal structure of E. coli RF3*GDP, which has a three-domain architecture strikingly similar to the structure of EF-Tu*GTP. Biochemical data on RF3 mutants show that a surface region involving domains II and III is important for distinct steps in the action cycle of RF3. Furthermore, we present a cryo-electron microscopy (cryo-EM) structure of the posttermination ribosome bound with RF3 in the GTP form. Our data show that RF3*GTP binding induces large conformational changes in the ribosome, which break the interactions of the class I RF with both the decoding center and the GTPase-associated center of the ribosome, apparently leading to the release of the class I RF.
About this StructureAbout this Structure
2O0F is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
RF3 induces ribosomal conformational changes responsible for dissociation of class I release factors., Gao H, Zhou Z, Rawat U, Huang C, Bouakaz L, Wang C, Cheng Z, Liu Y, Zavialov A, Gursky R, Sanyal S, Ehrenberg M, Frank J, Song H, Cell. 2007 Jun 1;129(5):929-41. PMID:17540173
Page seeded by OCA on Thu Mar 20 17:53:28 2008