2npx: Difference between revisions
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[[Image:2npx.jpg|left|200px]] | [[Image:2npx.jpg|left|200px]] | ||
'''NADH BINDING SITE AND CATALYSIS OF NADH PEROXIDASE''' | {{Structure | ||
|PDB= 2npx |SIZE=350|CAPTION= <scene name='initialview01'>2npx</scene>, resolution 2.4Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CYO:OXYGENS+BOUND+TO+CYS+SG'>CYO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> and <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/NADH_peroxidase NADH peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.1 1.11.1.1] | |||
|GENE= | |||
}} | |||
'''NADH BINDING SITE AND CATALYSIS OF NADH PEROXIDASE''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2NPX is a [ | 2NPX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NPX OCA]. | ||
==Reference== | ==Reference== | ||
NADH binding site and catalysis of NADH peroxidase., Stehle T, Claiborne A, Schulz GE, Eur J Biochem. 1993 Jan 15;211(1-2):221-6. PMID:[http:// | NADH binding site and catalysis of NADH peroxidase., Stehle T, Claiborne A, Schulz GE, Eur J Biochem. 1993 Jan 15;211(1-2):221-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8425532 8425532] | ||
[[Category: Enterococcus faecalis]] | [[Category: Enterococcus faecalis]] | ||
[[Category: NADH peroxidase]] | [[Category: NADH peroxidase]] | ||
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[[Category: oxidoreductase(h2o2(a))]] | [[Category: oxidoreductase(h2o2(a))]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:49:34 2008'' | ||
Revision as of 18:49, 20 March 2008
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| , resolution 2.4Å | |||||||
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| Ligands: | , and | ||||||
| Activity: | NADH peroxidase, with EC number 1.11.1.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NADH BINDING SITE AND CATALYSIS OF NADH PEROXIDASE
OverviewOverview
The structure of the complex between cofactor NADH and the enzyme NADH peroxidase from Streptococcus faecalis 10C1 (Enterococcus faecalis) has been determined by crystal soaking, X-ray data collection, model building of NADH and refinement at 0.24-nm resolution based on the known enzyme structure [Stehle, T., Ahmed, S. A., Claiborne, A. & Schulz, G. E. (1991) J. Mol. Biol. 221, 1325-1344]. Apart from NADH, the catalytic center of the enzyme contains FAD and a cysteine that shuttles between thiolate and sulfenic acid states. Unfortunately, this cysteine was irreversibly oxidized to a cysteine sulfonic acid in the established enzyme structure. Based on the geometry of the catalytic center, we discuss the stabilization of the oxidation-sensitive sulfenic acid and propose a reaction mechanism.
About this StructureAbout this Structure
2NPX is a Single protein structure of sequence from Enterococcus faecalis. Full crystallographic information is available from OCA.
ReferenceReference
NADH binding site and catalysis of NADH peroxidase., Stehle T, Claiborne A, Schulz GE, Eur J Biochem. 1993 Jan 15;211(1-2):221-6. PMID:8425532
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