1h3b: Difference between revisions
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==Overview== | ==Overview== | ||
The binding structures of 11 human oxidosqualene cyclase inhibitors, designed as cholesterol-lowering agents were determined for the, squalene-hopene cyclase from Alicyclobacillus acidocaldarius, which is the, only structurally known homologue of the human enzyme. The complexes were, produced by cocrystallization, and the structures were elucidated by X-ray, diffraction analyses. All inhibitors were bound in the large active center, cavity. The detailed binding structures are presented and discussed in the, light of the IC50 values of these 11 as well as 17 other inhibitors. They, provide a consistent picture for the inhibition of the bacterial enzyme, and can be used to adjust and improve homology models of the human enzyme., The detailed active center structures of the two enzymes are . | The binding structures of 11 human oxidosqualene cyclase inhibitors, designed as cholesterol-lowering agents were determined for the, squalene-hopene cyclase from Alicyclobacillus acidocaldarius, which is the, only structurally known homologue of the human enzyme. The complexes were, produced by cocrystallization, and the structures were elucidated by X-ray, diffraction analyses. All inhibitors were bound in the large active center, cavity. The detailed binding structures are presented and discussed in the, light of the IC50 values of these 11 as well as 17 other inhibitors. They, provide a consistent picture for the inhibition of the bacterial enzyme, and can be used to adjust and improve homology models of the human enzyme., The detailed active center structures of the two enzymes are too different, to show an IC50 correlation. | ||
==About this Structure== | ==About this Structure== | ||
1H3B is a | 1H3B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alicyclobacillus_acidocaldarius Alicyclobacillus acidocaldarius] with C8E and R46 as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: C8A. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H3B OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: oxidosqualene cyclase]] | [[Category: oxidosqualene cyclase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:01:37 2007'' | ||
Revision as of 14:56, 5 November 2007
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SQUALENE-HOPENE CYCLASE
OverviewOverview
The binding structures of 11 human oxidosqualene cyclase inhibitors, designed as cholesterol-lowering agents were determined for the, squalene-hopene cyclase from Alicyclobacillus acidocaldarius, which is the, only structurally known homologue of the human enzyme. The complexes were, produced by cocrystallization, and the structures were elucidated by X-ray, diffraction analyses. All inhibitors were bound in the large active center, cavity. The detailed binding structures are presented and discussed in the, light of the IC50 values of these 11 as well as 17 other inhibitors. They, provide a consistent picture for the inhibition of the bacterial enzyme, and can be used to adjust and improve homology models of the human enzyme., The detailed active center structures of the two enzymes are too different, to show an IC50 correlation.
About this StructureAbout this Structure
1H3B is a Single protein structure of sequence from Alicyclobacillus acidocaldarius with C8E and R46 as ligands. Structure known Active Site: C8A. Full crystallographic information is available from OCA.
ReferenceReference
Binding structures and potencies of oxidosqualene cyclase inhibitors with the homologous squalene-hopene cyclase., Lenhart A, Reinert DJ, Aebi JD, Dehmlow H, Morand OH, Schulz GE, J Med Chem. 2003 May 22;46(11):2083-92. PMID:12747780
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