2ccl: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2ccl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CCL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CCL FirstGlance]. <br> | <table><tr><td colspan='2'>[[2ccl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CCL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CCL FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>< | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1dyo|1dyo]], [[1gkk|1gkk]], [[1gkl|1gkl]], [[1h6x|1h6x]], [[1h6y|1h6y]], [[1ohz|1ohz]], [[1wb4|1wb4]], [[1wb5|1wb5]], [[1wb6|1wb6]], [[1anu|1anu]], [[1aoh|1aoh]], [[1nbc|1nbc]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1dyo|1dyo]], [[1gkk|1gkk]], [[1gkl|1gkl]], [[1h6x|1h6x]], [[1h6y|1h6y]], [[1ohz|1ohz]], [[1wb4|1wb4]], [[1wb5|1wb5]], [[1wb6|1wb6]], [[1anu|1anu]], [[1aoh|1aoh]], [[1nbc|1nbc]]</td></tr> | ||
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr> | ||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ccl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ccl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ccl RCSB], [http://www.ebi.ac.uk/pdbsum/2ccl PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ccl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ccl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ccl RCSB], [http://www.ebi.ac.uk/pdbsum/2ccl PDBsum]</span></td></tr> | ||
<table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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[[Category: Clostridium thermocellum]] | [[Category: Clostridium thermocellum]] | ||
[[Category: Endo-1,4-beta-xylanase]] | [[Category: Endo-1,4-beta-xylanase]] | ||
[[Category: Carvalho, A L | [[Category: Carvalho, A L]] | ||
[[Category: Davies, G J | [[Category: Davies, G J]] | ||
[[Category: Dias, F M.V | [[Category: Dias, F M.V]] | ||
[[Category: Ferreira, L M.A | [[Category: Ferreira, L M.A]] | ||
[[Category: Fontes, C M.G A | [[Category: Fontes, C M.G A]] | ||
[[Category: Gilbert, H J | [[Category: Gilbert, H J]] | ||
[[Category: Prates, J A.M | [[Category: Prates, J A.M]] | ||
[[Category: Romao, M J | [[Category: Romao, M J]] | ||
[[Category: Cell adhesion]] | [[Category: Cell adhesion]] | ||
[[Category: Cellulose degradation]] | [[Category: Cellulose degradation]] | ||
[[Category: Cellulosome]] | [[Category: Cellulosome]] | ||
[[Category: Cohesin]] | [[Category: Cohesin]] | ||
[[Category: Cohesin-dockerin complex]] | [[Category: Cohesin-dockerin complex]] | ||
Revision as of 20:52, 15 January 2015
THE S45A, T46A MUTANT OF THE TYPE I COHESIN-DOCKERIN COMPLEX FROM THE CELLULOSOME OF CLOSTRIDIUM THERMOCELLUMTHE S45A, T46A MUTANT OF THE TYPE I COHESIN-DOCKERIN COMPLEX FROM THE CELLULOSOME OF CLOSTRIDIUM THERMOCELLUM
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe assembly of proteins that display complementary activities into macromolecular complexes is critical to cellular function. One such enzyme complex, of environmental significance, is the plant cell wall degrading apparatus of anaerobic bacteria, termed the cellulosome. The complex assembles through the interaction of enzyme-derived "type I dockerin" modules with the multiple "cohesin" modules of the scaffolding protein. Clostridium thermocellum type I dockerin modules contain a duplicated 22-residue sequence that comprises helix-1 and helix-3, respectively. The crystal structure of a C. thermocellum type I cohesin-dockerin complex showed that cohesin recognition was predominantly through helix-3 of the dockerin. The sequence duplication is reflected in near-perfect 2-fold structural symmetry, suggesting that both repeats could interact with cohesins by a common mechanism in wild-type (WT) proteins. Here, a helix-3 disrupted mutant dockerin is used to visualize the reverse binding in which the dockerin mutant is indeed rotated 180 degrees relative to the WT dockerin such that helix-1 now dominates recognition of its protein partner. The dual binding mode is predicted to impart significant plasticity into the orientation of the catalytic subunits within this supramolecular assembly, which reflects the challenges presented by the degradation of a heterogeneous, recalcitrant, insoluble substrate by a tethered macromolecular complex. Evidence for a dual binding mode of dockerin modules to cohesins.,Carvalho AL, Dias FM, Nagy T, Prates JA, Proctor MR, Smith N, Bayer EA, Davies GJ, Ferreira LM, Romao MJ, Fontes CM, Gilbert HJ Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3089-94. Epub 2007 Feb 20. PMID:17360613[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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