2jnf: Difference between revisions
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'''Solution structure of fly troponin C, isoform F1''' | {{Structure | ||
|PDB= 2jnf |SIZE=350|CAPTION= <scene name='initialview01'>2jnf</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= tnC4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=212017 Lethocerus indicus]) | |||
}} | |||
'''Solution structure of fly troponin C, isoform F1''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2JNF is a [ | 2JNF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lethocerus_indicus Lethocerus indicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JNF OCA]. | ||
==Reference== | ==Reference== | ||
The structure of Lethocerus troponin C: insights into the mechanism of stretch activation in muscles., De Nicola G, Burkart C, Qiu F, Agianian B, Labeit S, Martin S, Bullard B, Pastore A, Structure. 2007 Jul;15(7):813-24. PMID:[http:// | The structure of Lethocerus troponin C: insights into the mechanism of stretch activation in muscles., De Nicola G, Burkart C, Qiu F, Agianian B, Labeit S, Martin S, Bullard B, Pastore A, Structure. 2007 Jul;15(7):813-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17637342 17637342] | ||
[[Category: Lethocerus indicus]] | [[Category: Lethocerus indicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: troponin c]] | [[Category: troponin c]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:43:12 2008'' | ||
Revision as of 18:43, 20 March 2008
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| Gene: | tnC4 (Lethocerus indicus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of fly troponin C, isoform F1
OverviewOverview
To gain a molecular description of how muscles can be activated by mechanical stretch, we have solved the structure of the calcium-loaded F1 isoform of troponin C (TnC) from Lethocerus and characterized its interactions with troponin I (TnI). We show that the presence of only one calcium cation in the fourth EF hand motif is sufficient to induce an open conformation in the C-terminal lobe of F1 TnC, in contrast with what is observed in vertebrate muscle. This lobe interacts in a calcium-independent way both with the N terminus of TnI and, with lower affinity, with a region of TnI equivalent to the switch and inhibitory peptides of vertebrate muscles. Using both synthetic peptides and recombinant proteins, we show that the N lobe of F1 TnC is not engaged in interactions with TnI, excluding a regulatory role of this domain. These findings provide insights into mechanically stimulated muscle contraction.
About this StructureAbout this Structure
2JNF is a Single protein structure of sequence from Lethocerus indicus. Full crystallographic information is available from OCA.
ReferenceReference
The structure of Lethocerus troponin C: insights into the mechanism of stretch activation in muscles., De Nicola G, Burkart C, Qiu F, Agianian B, Labeit S, Martin S, Bullard B, Pastore A, Structure. 2007 Jul;15(7):813-24. PMID:17637342
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