2jb5: Difference between revisions
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[[Image:2jb5.jpg|left|200px]] | [[Image:2jb5.jpg|left|200px]] | ||
'''FAB FRAGMENT IN COMPLEX WITH SMALL MOLECULE HAPTEN, CRYSTAL FORM-1''' | {{Structure | ||
|PDB= 2jb5 |SIZE=350|CAPTION= <scene name='initialview01'>2jb5</scene>, resolution 2.8Å | |||
|SITE= <scene name='pdbsite=AC1:T5c+Binding+Site+For+Chain+H'>AC1</scene> | |||
|LIGAND= <scene name='pdbligand=T5C:'>T5C</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''FAB FRAGMENT IN COMPLEX WITH SMALL MOLECULE HAPTEN, CRYSTAL FORM-1''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2JB5 is a [ | 2JB5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JB5 OCA]. | ||
==Reference== | ==Reference== | ||
Fab MOR03268 triggers absorption shift of a diagnostic dye via packaging in a solvent-shielded fab dimer interface., Hillig RC, Urlinger S, Fanghanel J, Brocks B, Haenel C, Stark Y, Sulzle D, Svergun DI, Baesler S, Malawski G, Moosmayer D, Menrad A, Schirner M, Licha K, J Mol Biol. 2008 Mar 14;377(1):206-19. Epub 2008 Jan 5. PMID:[http:// | Fab MOR03268 triggers absorption shift of a diagnostic dye via packaging in a solvent-shielded fab dimer interface., Hillig RC, Urlinger S, Fanghanel J, Brocks B, Haenel C, Stark Y, Sulzle D, Svergun DI, Baesler S, Malawski G, Moosmayer D, Menrad A, Schirner M, Licha K, J Mol Biol. 2008 Mar 14;377(1):206-19. Epub 2008 Jan 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18241888 18241888] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: tsc]] | [[Category: tsc]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:39:38 2008'' | ||
Revision as of 18:39, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
FAB FRAGMENT IN COMPLEX WITH SMALL MOLECULE HAPTEN, CRYSTAL FORM-1
OverviewOverview
Molecular interactions between near-IR fluorescent probes and specific antibodies may be exploited to generate novel smart probes for diagnostic imaging. Using a new phage display technology, we developed such antibody Fab fragments with subnanomolar binding affinity for tetrasulfocyanine, a near-IR in vivo imaging agent. Unexpectedly, some Fabs induced redshifts of the dye absorption peak of up to 44 nm. This is the largest shift reported for a biological system so far. Crystal structure determination and absorption spectroscopy in the crystal in combination with microcalorimetry and small-angle X-ray scattering in solution revealed that the redshift is triggered by formation of a Fab dimer, with tetrasulfocyanine being buried in a fully closed protein cavity within the dimer interface. The derived principle of shifting the absorption peak of a symmetric dye via packaging within a Fab dimer interface may be transferred to other diagnostic fluorophores, opening the way towards smart imaging probes that change their wavelength upon interaction with an antibody.
About this StructureAbout this Structure
2JB5 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Fab MOR03268 triggers absorption shift of a diagnostic dye via packaging in a solvent-shielded fab dimer interface., Hillig RC, Urlinger S, Fanghanel J, Brocks B, Haenel C, Stark Y, Sulzle D, Svergun DI, Baesler S, Malawski G, Moosmayer D, Menrad A, Schirner M, Licha K, J Mol Biol. 2008 Mar 14;377(1):206-19. Epub 2008 Jan 5. PMID:18241888
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