Sandbox Reserved 957: Difference between revisions

← Older edit Newer edit →

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA, Michael Pierrelee

@@ Line 38: / Line 38: @@
 * In particular the hydrophobic residues <scene name='60/604476/Lsite1/1'>Tyr664, Met816, Ala823, Gly819</scene>. [24]<br \>
-Besides, the kcat of the catalytic fragment decreases 40-fold and 8-fold if the residues His603 and Asp644 are mutated, and so there are important in the catalytic activity [5,3]. Two others residues are significant: the Gln778 which is important for cGMP affinity but have no impact on cAMP affinity H-loop [8] and the conserved Gly659 which is important for substrate affinity and catalytic activity because it determinates H-loop conformation [21] (see below).<br \>
+Besides, the kcat of the catalytic fragment decreases 40-fold and 8-fold if the residues <scene name='60/604476/Lys603_leu644/1'>His603 and Asp644</scene> are mutated, and so there are important in the catalytic activity [5,3]. Two others residues are significant: <scene name='60/604476/Gln778/1'>the Gln778</scene> which is important for cGMP affinity but have no impact on cAMP affinity H-loop [8] and the conserved <scene name='60/604476/Gly659/1'>Gly659</scene> which is important for substrate affinity and catalytic activity because it determinates H-loop conformation [21] (see below).<br \>
-H-loop is important in the substrate recognition and the interactions with, it is from 660th to 693th residues. H-loop has the same interactions with cGMP and Sildenafil (cf. Inhibitor) because it's related to its role of substrate binding[21]. But The H-loop is not well understood, because when it's modified, the enzyme's function is practically not modified.[7] But it also may have a role for inhibitor fixation.<br \>
+H-loop is important in the substrate recognition and the interactions with, it is <scene name='60/604476/H_loop/1'>from 660th to 693th residues</scene>. H-loop has the same interactions with cGMP and Sildenafil (cf. Inhibitor) because it's related to its role of substrate binding[21]. But The H-loop is not well understood, because when it's modified, the enzyme's function is practically not modified.[7] But it also may have a role for inhibitor fixation.<br \>
 Nowadays catalysis mechanism is not well know: there could be a, nucleophile attack of a water molecule on the substrate [6].