Sandbox Reserved 957: Difference between revisions
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H site:<br \> | H site:<br \> | ||
* In particular the residues Phe786, Ala783, Leu804, Val782. [24]<br \> | * In particular the residues <scene name='60/604476/Hsite1/1'>Phe786, Ala783, Leu804, Val782</scene>. [24]<br \> | ||
L site:<br \> | L site:<br \> | ||
* In particular the hydrophobic residues Tyr664, Met816, Ala823, Gly819. [24]<br \> | * In particular the hydrophobic residues <scene name='60/604476/Lsite1/1'>Tyr664, Met816, Ala823, Gly819</scene>. [24]<br \> | ||
Besides, the kcat of the catalytic fragment decreases 40-fold and 8-fold if the residues His603 and Asp644 are mutated, and so there are important in the catalytic activity [5,3]. Two others residues are significant: the Gln778 which is important for cGMP affinity but have no impact on cAMP affinity H-loop [8] and the conserved Gly659 which is important for substrate affinity and catalytic activity because it determinates H-loop conformation [21] (see below).<br \> | Besides, the kcat of the catalytic fragment decreases 40-fold and 8-fold if the residues His603 and Asp644 are mutated, and so there are important in the catalytic activity [5,3]. Two others residues are significant: the Gln778 which is important for cGMP affinity but have no impact on cAMP affinity H-loop [8] and the conserved Gly659 which is important for substrate affinity and catalytic activity because it determinates H-loop conformation [21] (see below).<br \> | ||