2j6v: Difference between revisions
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[[Image:2j6v.gif|left|200px]] | [[Image:2j6v.gif|left|200px]] | ||
'''CRYSTAL STRUCTURE OF THE DNA REPAIR ENZYME UV DAMAGE ENDONUC''' | {{Structure | ||
|PDB= 2j6v |SIZE=350|CAPTION= <scene name='initialview01'>2j6v</scene>, resolution 1.55Å | |||
|SITE= <scene name='pdbsite=AC1:Mn+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Po4+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Mn+Binding+Site+For+Chain+B'>AC3</scene> and <scene name='pdbsite=AC4:Po4+Binding+Site+For+Chain+B'>AC4</scene> | |||
|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF THE DNA REPAIR ENZYME UV DAMAGE ENDONUC''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2J6V is a [ | 2J6V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J6V OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of the DNA repair enzyme ultraviolet damage endonuclease., Paspaleva K, Thomassen E, Pannu NS, Iwai S, Moolenaar GF, Goosen N, Abrahams JP, Structure. 2007 Oct;15(10):1316-24. PMID:[http:// | Crystal structure of the DNA repair enzyme ultraviolet damage endonuclease., Paspaleva K, Thomassen E, Pannu NS, Iwai S, Moolenaar GF, Goosen N, Abrahams JP, Structure. 2007 Oct;15(10):1316-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17937920 17937920] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
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[[Category: uvde]] | [[Category: uvde]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:37:59 2008'' | ||
Revision as of 18:38, 20 March 2008
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| , resolution 1.55Å | |||||||
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| Sites: | , , and | ||||||
| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THE DNA REPAIR ENZYME UV DAMAGE ENDONUC
OverviewOverview
The ultraviolet damage endonuclease (UVDE) performs the initial step in an alternative excision repair pathway of UV-induced DNA damage, nicking immediately adjacent to the 5' phosphate of the damaged nucleotides. Unique for a single-protein DNA repair endonuclease, it can detect different types of damage. Here we show that Thermus thermophilus UVDE shares some essential structural features with Endo IV, an enzyme from the base excision repair pathway that exclusively nicks at abasic sites. A comparison between the structures indicates how DNA is bound by UVDE, how UVDE may recognize damage, and which of its residues are involved in catalysis. Furthermore, the comparison suggests an elegant explanation of UVDE's potential to recognize different types of damage. Incision assays including point mutants of UVDE confirmed the relevance of these conclusions.
About this StructureAbout this Structure
2J6V is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the DNA repair enzyme ultraviolet damage endonuclease., Paspaleva K, Thomassen E, Pannu NS, Iwai S, Moolenaar GF, Goosen N, Abrahams JP, Structure. 2007 Oct;15(10):1316-24. PMID:17937920
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