2j0m: Difference between revisions
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[[Image:2j0m.jpg|left|200px]] | [[Image:2j0m.jpg|left|200px]] | ||
'''CRYSTAL STRUCTURE A TWO-CHAIN COMPLEX BETWEEN THE FERM AND KINASE DOMAINS OF FOCAL ADHESION KINASE.''' | {{Structure | ||
|PDB= 2j0m |SIZE=350|CAPTION= <scene name='initialview01'>2j0m</scene>, resolution 2.80Å | |||
|SITE= <scene name='pdbsite=AC1:4st+Binding+Site+For+Chain+B'>AC1</scene> | |||
|LIGAND= <scene name='pdbligand=4ST:1,2,3,4-TETRAHYDROGEN-STAUROSPORINE'>4ST</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Non-specific_protein-tyrosine_kinase Non-specific protein-tyrosine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.2 2.7.10.2] | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE A TWO-CHAIN COMPLEX BETWEEN THE FERM AND KINASE DOMAINS OF FOCAL ADHESION KINASE.''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2J0M is a [ | 2J0M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J0M OCA]. | ||
==Reference== | ==Reference== | ||
Structural basis for the autoinhibition of focal adhesion kinase., Lietha D, Cai X, Ceccarelli DF, Li Y, Schaller MD, Eck MJ, Cell. 2007 Jun 15;129(6):1177-87. PMID:[http:// | Structural basis for the autoinhibition of focal adhesion kinase., Lietha D, Cai X, Ceccarelli DF, Li Y, Schaller MD, Eck MJ, Cell. 2007 Jun 15;129(6):1177-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17574028 17574028] | ||
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Non-specific protein-tyrosine kinase]] | [[Category: Non-specific protein-tyrosine kinase]] | ||
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[[Category: tyrosine-protein kinase]] | [[Category: tyrosine-protein kinase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:35:50 2008'' | ||
Revision as of 18:35, 20 March 2008
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| , resolution 2.80Å | |||||||
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| Sites: | |||||||
| Ligands: | |||||||
| Activity: | Non-specific protein-tyrosine kinase, with EC number 2.7.10.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE A TWO-CHAIN COMPLEX BETWEEN THE FERM AND KINASE DOMAINS OF FOCAL ADHESION KINASE.
OverviewOverview
Appropriate tyrosine kinase signaling depends on coordinated sequential coupling of protein-protein interactions with catalytic activation. Focal adhesion kinase (FAK) integrates signals from integrin and growth factor receptors to regulate cellular responses including cell adhesion, migration, and survival. Here, we describe crystal structures representing both autoinhibited and active states of FAK. The inactive structure reveals a mechanism of inhibition in which the N-terminal FERM domain directly binds the kinase domain, blocking access to the catalytic cleft and protecting the FAK activation loop from Src phosphorylation. Additionally, the FERM domain sequesters the Tyr397 autophosphorylation and Src recruitment site, which lies in the linker connecting the FERM and kinase domains. The active phosphorylated FAK kinase adopts a conformation that is immune to FERM inhibition. Our biochemical and structural analysis shows how the architecture of autoinhibited FAK orchestrates an activation sequence of FERM domain displacement, linker autophosphorylation, Src recruitment, and full catalytic activation.
About this StructureAbout this Structure
2J0M is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for the autoinhibition of focal adhesion kinase., Lietha D, Cai X, Ceccarelli DF, Li Y, Schaller MD, Eck MJ, Cell. 2007 Jun 15;129(6):1177-87. PMID:17574028
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