2ixr: Difference between revisions

Revision as of 18:34, 20 March 2008

, resolution 2.6Å

Coordinates:

BIPD OF BURKHOLDERIA PSEUDOMALLEI

OverviewOverview

Bacteria expressing type III secretion systems (T3SS) have been responsible for the deaths of millions worldwide, acting as key virulence elements in diseases ranging from plague to typhoid fever. The T3SS is composed of a basal body, which traverses both bacterial membranes, and an external needle through which effector proteins are secreted. We report multiple crystal structures of two proteins that sit at the tip of the needle and are essential for virulence: IpaD from Shigella flexneri and BipD from Burkholderia pseudomallei. The structures reveal that the N-terminal domains of the molecules are intramolecular chaperones that prevent premature oligomerization, as well as sharing structural homology with proteins involved in eukaryotic actin rearrangement. Crystal packing has allowed us to construct a model for the tip complex that is supported by mutations designed using the structure.

About this StructureAbout this Structure

2IXR is a Single protein structure of sequence from Burkholderia pseudomallei. Full crystallographic information is available from OCA.

ReferenceReference

Self-chaperoning of the type III secretion system needle tip proteins IpaD and BipD., Johnson S, Roversi P, Espina M, Olive A, Deane JE, Birket S, Field T, Picking WD, Blocker AJ, Galyov EE, Picking WL, Lea SM, J Biol Chem. 2007 Feb 9;282(6):4035-44. Epub 2006 Oct 31. PMID:17077085

Page seeded by OCA on Thu Mar 20 17:34:45 2008

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OCA

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-[[Image:2ixr.jpg|left|200px]]<br /><applet load="2ixr" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2ixr.jpg|left|200px]]
-caption="2ixr, resolution 2.6&Aring;" />
-'''BIPD OF BURKHOLDERIA PSEUDOMALLEI'''<br />
+ {{Structure
+|PDB= 2ixr |SIZE=350|CAPTION= <scene name='initialview01'>2ixr</scene>, resolution 2.6&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''BIPD OF BURKHOLDERIA PSEUDOMALLEI'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-IXR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IXR OCA].
+IXR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IXR OCA].
 ==Reference==
-Self-chaperoning of the type III secretion system needle tip proteins IpaD and BipD., Johnson S, Roversi P, Espina M, Olive A, Deane JE, Birket S, Field T, Picking WD, Blocker AJ, Galyov EE, Picking WL, Lea SM, J Biol Chem. 2007 Feb 9;282(6):4035-44. Epub 2006 Oct 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17077085 17077085]
+Self-chaperoning of the type III secretion system needle tip proteins IpaD and BipD., Johnson S, Roversi P, Espina M, Olive A, Deane JE, Birket S, Field T, Picking WD, Blocker AJ, Galyov EE, Picking WL, Lea SM, J Biol Chem. 2007 Feb 9;282(6):4035-44. Epub 2006 Oct 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17077085 17077085]
 [[Category: Burkholderia pseudomallei]]
 [[Category: Single protein]]
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 [[Category: type 3 secretion system]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:57:10 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:34:45 2008''