2iwr: Difference between revisions

Revision as of 18:34, 20 March 2008

, resolution 1.50Å

Coordinates:

GTPASE LIKE DOMAIN OF CENTAURIN GAMMA 1 (HUMAN)

OverviewOverview

Centaurins are a family of proteins that contain GTPase-activating protein domains, with the gamma family members containing in addition a GTPase-like domain. Centaurins reside mainly in the nucleus and are known to activate phosphoinositide 3-kinase, a key regulator of cell proliferation, motility and vesicular trafficking. In the present study, using X-ray structural analysis, enzymatic assays and nucleotide-binding studies, we show that, for CENTG1 (centaurin gamma-1) the GTPase-like domain has broader trinucleotide specificity. Alterations within the G4 motif of CENTG1 from the highly conserved NKXD found in typical GTPases to TQDR result in the loss of specificity, a lower affinity for the nucleotides and higher turnover rates. These results indicate that the centaurins could be more accurately classified as NTPases and point to alternative mechanisms of cell signalling control.

About this StructureAbout this Structure

2IWR is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

The centaurin gamma-1 GTPase-like domain functions as an NTPase., Soundararajan M, Yang X, Elkins JM, Sobott F, Doyle DA, Biochem J. 2007 Feb 1;401(3):679-88. PMID:17037982

Page seeded by OCA on Thu Mar 20 17:34:20 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:2iwr.gif|left|200px]]<br /><applet load="2iwr" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2iwr.gif|left|200px]]
-caption="2iwr, resolution 1.50&Aring;" />
-'''GTPASE LIKE DOMAIN OF CENTAURIN GAMMA 1 (HUMAN)'''<br />
+ {{Structure
+|PDB= 2iwr |SIZE=350|CAPTION= <scene name='initialview01'>2iwr</scene>, resolution 1.50&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''GTPASE LIKE DOMAIN OF CENTAURIN GAMMA 1 (HUMAN)'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-IWR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IWR OCA].
+IWR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IWR OCA].
 ==Reference==
-The centaurin gamma-1 GTPase-like domain functions as an NTPase., Soundararajan M, Yang X, Elkins JM, Sobott F, Doyle DA, Biochem J. 2007 Feb 1;401(3):679-88. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17037982 17037982]
+The centaurin gamma-1 GTPase-like domain functions as an NTPase., Soundararajan M, Yang X, Elkins JM, Sobott F, Doyle DA, Biochem J. 2007 Feb 1;401(3):679-88. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17037982 17037982]
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
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 [[Category: sgc]]
 [[Category: structual genomics consortium]]
-[[Category: structural genomics]]
+[[Category: structural genomic]]
 [[Category: structural genomics consortium]]
 [[Category: transport]]
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 [[Category: zinc-finger]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:56:47 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:34:20 2008''