2iwm: Difference between revisions
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'''PRECURSOR MUTANT CYS1SER OF PENICILLIN V ACYLASE FROM BACILLUS SPHAERICUS''' | {{Structure | ||
|PDB= 2iwm |SIZE=350|CAPTION= <scene name='initialview01'>2iwm</scene>, resolution 2.50Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] | |||
|GENE= | |||
}} | |||
'''PRECURSOR MUTANT CYS1SER OF PENICILLIN V ACYLASE FROM BACILLUS SPHAERICUS''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2IWM is a [ | 2IWM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lysinibacillus_sphaericus Lysinibacillus sphaericus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IWM OCA]. | ||
==Reference== | ==Reference== | ||
Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants., Chandra PM, Brannigan JA, Prabhune A, Pundle A, Turkenburg JP, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt, 1):124-7. Epub 2004 Dec 24. PMID:[http:// | Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants., Chandra PM, Brannigan JA, Prabhune A, Pundle A, Turkenburg JP, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt, 1):124-7. Epub 2004 Dec 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16508111 16508111] | ||
[[Category: Lysinibacillus sphaericus]] | [[Category: Lysinibacillus sphaericus]] | ||
[[Category: Penicillin amidase]] | [[Category: Penicillin amidase]] | ||
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[[Category: zymogen]] | [[Category: zymogen]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:34:17 2008'' | ||
Revision as of 18:34, 20 March 2008
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| , resolution 2.50Å | |||||||
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| Activity: | Penicillin amidase, with EC number 3.5.1.11 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
PRECURSOR MUTANT CYS1SER OF PENICILLIN V ACYLASE FROM BACILLUS SPHAERICUS
OverviewOverview
The crystallization of three catalytically inactive mutants of penicillin V acylase (PVA) from Bacillus sphaericus in precursor and processed forms is reported. The mutant proteins crystallize in different primitive monoclinic space groups that are distinct from the crystal forms for the native enzyme. Directed mutants and clone constructs were designed to study the post-translational autoproteolytic processing of PVA. The catalytically inactive mutants will provide three-dimensional structures of precursor PVA forms, plus open a route to the study of enzyme-substrate complexes for this industrially important enzyme.
About this StructureAbout this Structure
2IWM is a Single protein structure of sequence from Lysinibacillus sphaericus. Full crystallographic information is available from OCA.
ReferenceReference
Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants., Chandra PM, Brannigan JA, Prabhune A, Pundle A, Turkenburg JP, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt, 1):124-7. Epub 2004 Dec 24. PMID:16508111
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