2iu4: Difference between revisions

← Older edit Newer edit →

Revision as of 18:33, 20 March 2008

File:2iu4.gif

, resolution 1.96Å

Sites:

Ligands:

and

Activity:

Glucokinase, with EC number 2.7.1.2

Coordinates:

save as pdb, mmCIF, xml

DIHYDROXYACETONE KINASE OPERON CO-ACTIVATOR DHA-DHAQ

OverviewOverview

Dihydroxyacetone (Dha) kinases are a novel family of kinases with signaling and metabolic functions. Here we report the x-ray structures of the transcriptional activator DhaS and the coactivator DhaQ and characterize their function. DhaQ is a paralog of the Dha binding Dha kinase subunit; DhaS belongs to the family of TetR repressors although, unlike all known members of this family, it is a transcriptional activator. DhaQ and DhaS form a stable complex that in the presence of Dha activates transcription of the Lactococcus lactis dha operon. Dha covalently binds to DhaQ through a hemiaminal bond with a histidine and thereby induces a conformational change, which is propagated to the surface via a cantilever-like structure. DhaS binding protects an inverted repeat whose sequence is GGACACATN6ATTTGTCC and renders two GC base pairs of the operator DNA hypersensitive to DNase I cleavage. The proximal half-site of the inverted repeat partially overlaps with the predicted -35 consensus sequence of the dha promoter.

About this StructureAbout this Structure

2IU4 is a Single protein structure of sequence from Lactococcus lactis. Full crystallographic information is available from OCA.

ReferenceReference

Regulation of the Dha operon of Lactococcus lactis: a deviation from the rule followed by the Tetr family of transcription regulators., Christen S, Srinivas A, Bahler P, Zeller A, Pridmore D, Bieniossek C, Baumann U, Erni B, J Biol Chem. 2006 Aug 11;281(32):23129-37. Epub 2006 Jun 7. PMID:16760471

Page seeded by OCA on Thu Mar 20 17:33:23 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2iu4.gif|left|200px]]<br /><applet load="2iu4" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2iu4.gif|left|200px]]
-caption="2iu4, resolution 1.96&Aring;" />
-'''DIHYDROXYACETONE KINASE OPERON CO-ACTIVATOR DHA-DHAQ'''<br />
+ {{Structure
+|PDB= 2iu4 |SIZE=350|CAPTION= <scene name='initialview01'>2iu4</scene>, resolution 1.96&Aring;
+|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+B'>AC1</scene>
+|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=HIQ:1-[1,2-DIHYDROXY-1-(HYDROXYMETHYL)ETHYL]-L-HISTIDINE'>HIQ</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2]
+|GENE=
+}}
+'''DIHYDROXYACETONE KINASE OPERON CO-ACTIVATOR DHA-DHAQ'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-IU4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=HIQ:'>HIQ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] Known structural/functional Site: <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IU4 OCA].
+IU4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IU4 OCA].
 ==Reference==
-Regulation of the Dha operon of Lactococcus lactis: a deviation from the rule followed by the Tetr family of transcription regulators., Christen S, Srinivas A, Bahler P, Zeller A, Pridmore D, Bieniossek C, Baumann U, Erni B, J Biol Chem. 2006 Aug 11;281(32):23129-37. Epub 2006 Jun 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16760471 16760471]
+Regulation of the Dha operon of Lactococcus lactis: a deviation from the rule followed by the Tetr family of transcription regulators., Christen S, Srinivas A, Bahler P, Zeller A, Pridmore D, Bieniossek C, Baumann U, Erni B, J Biol Chem. 2006 Aug 11;281(32):23129-37. Epub 2006 Jun 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16760471 16760471]
 [[Category: Glucokinase]]
 [[Category: Lactococcus lactis]]
@@ Line 23: / Line 32: @@
 [[Category: dihydroxyacetone]]
 [[Category: kinase]]
-[[Category: lactococcus lactis]]
+[[Category: lactococcus lacti]]
 [[Category: transferase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:56:00 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:33:23 2008''